Assessment of the in vitro bioactive properties of lactic acid bacteria isolated from native ecological niches of Ecuador
| Autor: | Ana B. Benavides, Gabriela N. Tenea, Lucia Yépez, Mario Ulcuango |
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| Rok vydání: | 2016 |
| Předmět: |
Bacterias ácido lácticas
0301 basic medicine Microbiology (medical) Rainforest Potencial probiótico 030106 microbiology Flowers Microbial Sensitivity Tests Actividad antimicrobiana Antimicrobial activity Microbiology 03 medical and health sciences chemistry.chemical_compound Species Specificity Antibiosis Lactic acid bacteria medicine Food microbiology biology Probiotics Temperature Drug Resistance Microbial Kanamycin Salt Tolerance General Medicine Lactobacillaceae Plants biology.organism_classification Antimicrobial Bacterial Typing Techniques Random Amplified Polymorphic DNA Technique Lactic acid Phenotype 030104 developmental biology Probiotic potential chemistry Fruit Food-borne pathogens Fermentation Food Microbiology Ecuador Patógenos alimentarios Bacteria medicine.drug |
| Zdroj: | Revista Argentina de Microbiología. 48:236-244 |
| ISSN: | 0325-7541 |
| DOI: | 10.1016/j.ram.2016.05.003 |
| Popis: | Lactic acid bacteria are known for their biotechnological potential. In various regions of Ecuador numerous indigenous biological resources are largely undocumented. In this study, we evaluated the potential probiotic characteristics and antagonistic in vitro properties of some lactic acid bacteria from native niches of the subtropical rain forests of Ecuador. These isolates were identified according to their morphological properties, standard API50CH fermentation profile and RAPD-DNA polymorphism pattern. The selected isolates were further evaluated for their probiotic potential. The isolates grew at 15°C and 45°C, survived at a pH ranging from 2.5 to 4.5 in the presence of 0.3% bile (>90%) and grew under sodium chloride conditions. All selected isolates were sensitive to ampicillin, amoxicillin and cefuroxime and some showed resistance to gentamicin, kanamycin and tetracycline. Moreover, the agar well diffusion assay showed that the supernatant of each strain at pH 3.0 and pH 4.0, but not at pH 7.0 exhibited increased antimicrobial activity (inhibition zone >15mm) against two foodborne pathogens, Escherichia coli and Salmonella spp. To our knowledge, this is the first report describing the antagonistic activity against two foodborne pathogens and the probiotic in vitro potential of lactic acid bacteria isolated from native biota of Ecuador. |
| Databáze: | OpenAIRE |
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