Successful use of oviduct epithelial cell coculture for in vitro production of viable red deer (Cervus elaphus) embryos

Autor: Yves Cognié, N. Poulin, Gérard Baril, M. Verdier, X. Legendre, J. C. Vallet, Pascal Mermillod, Yann Locatelli
Přispěvatelé: Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Unité de Recherches sur les Herbivores (URH), Institut National de la Recherche Agronomique (INRA), Direction des Jardins botaniques et zoologiques, Muséum national d'Histoire naturelle (MNHN), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2005
Předmět:
animal structures
[SDV]Life Sciences [q-bio]
Embryonic Development
Fertilization in Vitro
Biology
cryopreservation
Cryopreservation
Embryo Culture Techniques
Andrology
03 medical and health sciences
0302 clinical medicine
Human fertilization
wild specie
Food Animals
Pregnancy
medicine
Animals
[INFO]Computer Science [cs]
Blastocyst
Small Animals
Fallopian Tubes
reproductive and urinary physiology
2. Zero hunger
030219 obstetrics & reproductive medicine
offspring
blastocyst
urogenital system
Equine
Deer
Embryogenesis
0402 animal and dairy science
Epithelial Cells
Embryo
04 agricultural and veterinary sciences
Anatomy
Embryo Transfer
Oocyte
040201 dairy & animal science
Coculture Techniques
In vitro maturation
medicine.anatomical_structure
fertilization
embryonic structures
Oviduct
Female
Animal Science and Zoology
transfer
Zdroj: Theriogenology
Theriogenology, Elsevier, 2005, 64, pp.1729-1739. ⟨10.1016/j.theriogenology.2005.04.002⟩
ISSN: 0093-691X
DOI: 10.1016/j.theriogenology.2005.04.002
Popis: International audience; Techniques for in vitro production (IVP) of viable embryos have been thoroughly developed in several domestic species in view to improve breeding efficiency. When applied to wild life, these techniques may also help the maintenance of biodiversity through amplification of sparse animals offspring and facilitation of genetic material exchange. During the successive steps of IVP, i.e. oocyte in vitro maturation (IVM), fertilization (IVF) and early embryo development (IVD) to the blastocyst stage, gametes and embryos are faced with unusual environment, including oxidative stress, known to be detrimental to their survival. In the present study, starting from methods developed in domestic species, we have adapted IVP to produce viable red deer embryos. In a first experiment, cumulus cells were removed from in vitro matured oocytes either before or after IVF. The presence of cumulus cells during IVF did not affect final cleavage or development rates. In a second experiment, in vitro matured oocytes were fertilized in the presence of cumulus cells and cultured in SOFaaBSA medium alone or in the presence of ovine oviduct epithelial cell (oOEC) monolayer. Whereas, oviduct cells did not improve the cleavage rate, they significantly increased the rate of embryos reaching the blastocyst stage (from 3 to 25% of total oocytes). Ten blastocysts from oOEC coculture were transferred after freezing and thawing to five recipient hinds and gave rise to three pregnancies. The three pregnant hinds gave birth to three live and normal calves.
Databáze: OpenAIRE
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