Pectin as Carbon Source for Monilinia laxa Exoproteome and Expression Profiles of Related Genes
| Autor: | Silvia Rodríguez-Pires, Paloma Melgarejo, Eduardo A. Espeso, Antonieta De Cal |
|---|---|
| Přispěvatelé: | Ministerio de Ciencia e Innovación (España), Ministerio de Ciencia, Innovación y Universidades (España), Rodríguez-Pires, Silvia [0000-0001-7482-1715], Melgarejo, Paloma [0000-0002-3698-8896], Cal, Antonieta de [0000-0002-7725-7782], Espeso, Eduardo A. [0000-0002-5873-6059], Rodríguez-Pires, Silvia, Melgarejo, Paloma, Cal, Antonieta de, Espeso, Eduardo A. |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine animal structures food.ingredient Pectin Physiology In silico macromolecular substances complex mixtures 01 natural sciences Cell wall Necrotroph 03 medical and health sciences food Extracellular Secretion Pectinase Gene Aterosclerosis biology digestive oral and skin physiology food and beverages Pectin degradation General Medicine biology.organism_classification Cell wall degrading enzymes 030104 developmental biology Biochemistry Agronomy and Crop Science CAZymes Monilinia laxa 010606 plant biology & botany |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 1943-7706 0894-0282 |
| DOI: | 10.1094/mpmi-01-20-0019-r |
| Popis: | 73 p.-6 fig.-2 tab.-1 fig. supl.-4 tab.supl. Pectin, as part of the fruit cell wall, can be degraded by brown rot fungi by coordinating the production, secretion, and action of extracellular enzymes. In this study, pectin utilization by the necrotroph Monilinia laxa 8L was studied by in vitro and in silico approaches. A total of 403 genes encoding carbohydrate-active enzymes (CAZymes) were identified, including 38 coding a predicted pectin-degrading activity. Analyzing the differences between M. laxa 8L exoproteomes in media containing glucose and pectin as sole carbon sources, we identified 107 pectin-specific proteins, among them, 64.48% harbor a classical secretory activity, including 42 CAZymes and six pectin-degrading proteins. Analyzing the gene-expression patterns of some pectinase families revealed their possible sequential action in pectin disassembly. We found, in vitro, an early pectin-dependent induction of MlRGAE1, MlPG1, and three members of the rhamnosidase family (MlαRHA2, MlαRHA3, and MlαRHA6) and late response of MlPG2 and MlPNL3. M. laxa 8L has the ability to use both pectin and byproducts as carbon sources, based on a functional pectinolytic machinery encoded in its genome, subjected to pectin-dependent regulation and appropriate secretion mechanisms of these pectinolytic enzymes. Differences in the secretion and transcription profile of M. laxa 8L provided insights into the different mechanisms that contribute to brown rot development. Ministry of Science, Innovation and Universities (MCIU, Spain), Agencia Estatal de Investigación (AEI) and the Fondo Europeo de Desarrollo Regional (FEDER, EU): AGL2014-55287-C2-1-R, BFU2015-66806-R, RTI2018- 094263-B-I00 and AGL2017-84389-C2-2-R |
| Databáze: | OpenAIRE |
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