Evaluation of RT-qPCR of mouthwash and buccal swabs for detection of SARS-CoV-2 in children and adults
| Autor: | Christoph Wenisch, Tamara Seitz, Franz Allerberger, Hasan Kelani, Hermann Laferl, Thomas Frischer, Sebastian Baier-Grabner, Florian Götzinger, Florian Heger, Elisabeth Scholz |
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| Rok vydání: | 2022 |
| Předmět: |
Adult
buccal swab 2019-20 coronavirus outbreak Coronavirus disease 2019 (COVID-19) Epidemiology Specimen Collection Method Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Buccal swab Mouthwashes Dentistry Specimen Handling stomatognathic system Nasopharynx Major Article Humans Medicine In patient Child Collection methods SARS-CoV-2 business.industry Health Policy Public Health Environmental and Occupational Health COVID-19 body regions Infectious Diseases mouth wash Detection rate business |
| Zdroj: | American Journal of Infection Control |
| ISSN: | 0196-6553 |
| DOI: | 10.1016/j.ajic.2021.10.019 |
| Popis: | Background The use of nasopharyngeal (NP) swabs as a specimen collection method to diagnose SARS-CoV-2 infection is frequently perceived as uncomfortable by patients and requires trained personnel. In this study, detection rate of SARS-CoV-2 in mouthwash samples and buccal swabs were compared in both children and adults. Material and methods In patients admitted to hospital with confirmed COVID-19 within the previous 72 hours, NP and buccal swabs as well as mouthwash samples were collected. RT-qPCR was performed on all samples. Results In total, 170 samples were collected from 155 patients (137 adults and 18 children). Approximately 91.7% of the collected NP swabs were positive in RT-PCR compared to 63.1% of mouthwash samples and 42.4% of buccal swabs. Compared to NP swabs, the sensitivity of using mouthwash was 96.3% and 65.4% for buccal swabs in NP swab samples with a CT value |
| Databáze: | OpenAIRE |
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