Microplate assay of glutathione s-transferase activity for resistance detection in single-mosquito triturates
| Autor: | William G. Brogdon, Ann M. Barber |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Physiology
Drug Evaluation Preclinical Drug Resistance Biology Biochemistry Toxicology Glutathione transferase chemistry.chemical_compound DDT resistance Species Specificity Anopheles parasitic diseases Anopheles arabiensis Animals Molecular Biology Analysis method Glutathione Transferase chemistry.chemical_classification General Medicine Glutathione Molecular biology Enzyme chemistry Insecticide resistance Larva Female Glutathione S-transferase activity |
| Zdroj: | Comparative Biochemistry and Physiology Part B: Comparative Biochemistry. 96:339-342 |
| ISSN: | 0305-0491 |
| Popis: | 1. 1. Optimum conditions are described for a simple, rapid microplate assay that measures glutathion s-transferase (GST) activity accurately and precisely in small portions of single mosquito homogenates. 2. 2. Up to 10 assay replicates were possible for individual adults and larvae. Concentration of GST activity in the head/thorax region allows blood-fed mosquitoes with abdomens removed to be used in assays. 3. 3. The method allows the use of GST activity as a biochemical character in comparative studies of populations. 4. 4. The microplate assay detects elevated GST activities associated with DDT resistance in Anopheles arabiensis. |
| Databáze: | OpenAIRE |
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