A latex agglutination assay for specific detection of Panton–Valentine leukocidin
| Autor: | Teruyo Ito, Kanenari Oishi, Tadashi Baba, Yasuo Nakatomi, Keiichi Hiramatsu |
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| Rok vydání: | 2008 |
| Předmět: |
Microbiology (medical)
Staphylococcus aureus Micrococcaceae Bacterial Toxins Leukocidin Exotoxins Biology medicine.disease_cause Sensitivity and Specificity Microbiology Leukocidins medicine Animals Humans skin and connective tissue diseases Molecular Biology Toxin Staphylococcal Infections respiratory system biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification Virology Latex fixation test Agglutination (biology) bacteria Rabbits Panton–Valentine leukocidin Latex Fixation Tests Bacteria |
| Zdroj: | Journal of Microbiological Methods. 75:411-415 |
| ISSN: | 0167-7012 |
| Popis: | Panton-Valentine leukocidin (PVL) is produced by some isolates of Staphylococcus aureus, and has been associated with the high pathogenic potential of these strains. To rapidly detect the toxin producer strains, we developed a reverse passive latex agglutination (RPLA) reaction assay specific for PVL. By testing 64 S. aureus strains, the assay could detect the 35 pvl-gene-positive strains with 100% specificity and sensitivity. Furthermore, the assay revealed an extensive variation in the amount of PVL produced by the pvl-positive strains. |
| Databáze: | OpenAIRE |
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