Analyses of the Temporal Dynamics of Fungal Communities Colonizing the Healthy Wood Tissues of Esca Leaf-Symptomatic and Asymptomatic Vines
Autor: | Patrice Rey, Jessica Vallance, Jean-Pierre Da Costa, Pascal Lecomte, Lucia Guerin-Dubrana, Emilie Bruez, Jonathan Gerbore |
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Přispěvatelé: | Unité Mixte de Recherche en Santé Végétale (INRA/ENITA) (UMRSV), Institut National de la Recherche Agronomique (INRA)-École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Institut des Sciences de la Vigne et du Vin (ISVV), Laboratoire de l'intégration, du matériau au système (IMS), Université Sciences et Technologies - Bordeaux 1-Institut Polytechnique de Bordeaux-Centre National de la Recherche Scientifique (CNRS), Santé et agroécologie du vignoble (UMR SAVE), Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro), Bordeaux Sciences Agro, Regional Council of Aquitaine, French Ministry of Agriculture, Food-processing industry and Forest [CASDAR V906], BIOVITIS, Unité Mixte de Recherche en Santé Végétale (INRA/ENITA) (UMR SAVE) |
Jazyk: | angličtina |
Rok vydání: | 2014 |
Předmět: |
0106 biological sciences
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences [SDV]Life Sciences [q-bio] Hypocreaceae lcsh:Medicine Plant Science contrôle de maladie 01 natural sciences Vitis DNA Fungal lcsh:Science 0303 health sciences Multidisciplinary esca de la vigne biology Microbiota food and beverages Agriculture Biodiversity Botryosphaeriaceae Wood Sampling time medicine.symptom Rootstock Research Article Plant Pathogens Crops Mycology Microbiology Asymptomatic 03 medical and health sciences Integrated Control vitis vinifera pcr Botany medicine Plant Diseases 030304 developmental biology lcsh:R extraction adn Fungi Organisms Biology and Life Sciences Crop Diseases Plant Pathology 15. Life on land biology.organism_classification maladie du bois de la vigne Plant Leaves Haplotypes White rot lcsh:Q Pest Control Viticulture diagnostic foliaire 010606 plant biology & botany |
Zdroj: | PLoS ONE PLoS ONE, Public Library of Science, 2014, 9 (5), pp.e95928 PLoS ONE, Public Library of Science, 2014, 9 (5), 15 p. ⟨10.1371/journal.pone.0095928⟩ Plos One 5 (9), 15 p.. (2014) PLoS ONE, Vol 9, Iss 5, p e95928 (2014) |
ISSN: | 1932-6203 |
DOI: | 10.1371/journal.pone.0095928⟩ |
Popis: | International audience; Esca, a Grapevine Trunk Disease (GTD), is of major concern for viticulture worldwide. Our study compares the fungal communities that inhabit the wood tissues of vines that expressed or not foliar esca-symptoms. The trunk and rootstock tissues were apparently healthy, whether the 10 year-old plants were symptomatic or not. The only difference was in the cordon, which contained white rot, a typical form of esca, in 79% of symptomatic plants. Observations over a period of one year using a fingerprint method, Single Strand Conformation Polymorphism (SSCP), and the ITS-DNA sequencing of cultivable fungi, showed that shifts occurred in the fungal communities colonizing the healthy wood tissues. However, whatever the sampling time, spring, summer, autumn or winter, the fungi colonizing the healthy tissues of asymptomatic or symptomatic plants were not significantly different. Forty-eight genera were isolated, with species of Hypocreaceae and Botryosphaeriaceae being the most abundant species. Diverse fungal assemblages, made up of potentially plant-pathogenic and -protective fungi, colonized these non-necrotic tissues. Some fungi, possibly involved in GTD, inhabited the non-necrotic wood of young plants, but no increase in necrosis areas was observed over the one-year period. |
Databáze: | OpenAIRE |
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