Analyses of the Temporal Dynamics of Fungal Communities Colonizing the Healthy Wood Tissues of Esca Leaf-Symptomatic and Asymptomatic Vines

Autor: Patrice Rey, Jessica Vallance, Jean-Pierre Da Costa, Pascal Lecomte, Lucia Guerin-Dubrana, Emilie Bruez, Jonathan Gerbore
Přispěvatelé: Unité Mixte de Recherche en Santé Végétale (INRA/ENITA) (UMRSV), Institut National de la Recherche Agronomique (INRA)-École Nationale d'Ingénieurs des Travaux Agricoles - Bordeaux (ENITAB)-Institut des Sciences de la Vigne et du Vin (ISVV), Laboratoire de l'intégration, du matériau au système (IMS), Université Sciences et Technologies - Bordeaux 1-Institut Polytechnique de Bordeaux-Centre National de la Recherche Scientifique (CNRS), Santé et agroécologie du vignoble (UMR SAVE), Institut National de la Recherche Agronomique (INRA)-Université de Bordeaux (UB)-Institut des Sciences de la Vigne et du Vin (ISVV)-Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine (Bordeaux Sciences Agro), Bordeaux Sciences Agro, Regional Council of Aquitaine, French Ministry of Agriculture, Food-processing industry and Forest [CASDAR V906], BIOVITIS, Unité Mixte de Recherche en Santé Végétale (INRA/ENITA) (UMR SAVE)
Jazyk: angličtina
Rok vydání: 2014
Předmět:
0106 biological sciences
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences
[SDV]Life Sciences [q-bio]
Hypocreaceae
lcsh:Medicine
Plant Science
contrôle de maladie
01 natural sciences
Vitis
DNA
Fungal

lcsh:Science
0303 health sciences
Multidisciplinary
esca de la vigne
biology
Microbiota
food and beverages
Agriculture
Biodiversity
Botryosphaeriaceae
Wood
Sampling time
medicine.symptom
Rootstock
Research Article
Plant Pathogens
Crops
Mycology
Microbiology
Asymptomatic
03 medical and health sciences
Integrated Control
vitis vinifera
pcr
Botany
medicine
Plant Diseases
030304 developmental biology
lcsh:R
extraction adn
Fungi
Organisms
Biology and Life Sciences
Crop Diseases
Plant Pathology
15. Life on land
biology.organism_classification
maladie du bois de la vigne
Plant Leaves
Haplotypes
White rot
lcsh:Q
Pest Control
Viticulture
diagnostic foliaire
010606 plant biology & botany
Zdroj: PLoS ONE
PLoS ONE, Public Library of Science, 2014, 9 (5), pp.e95928
PLoS ONE, Public Library of Science, 2014, 9 (5), 15 p. ⟨10.1371/journal.pone.0095928⟩
Plos One 5 (9), 15 p.. (2014)
PLoS ONE, Vol 9, Iss 5, p e95928 (2014)
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0095928⟩
Popis: International audience; Esca, a Grapevine Trunk Disease (GTD), is of major concern for viticulture worldwide. Our study compares the fungal communities that inhabit the wood tissues of vines that expressed or not foliar esca-symptoms. The trunk and rootstock tissues were apparently healthy, whether the 10 year-old plants were symptomatic or not. The only difference was in the cordon, which contained white rot, a typical form of esca, in 79% of symptomatic plants. Observations over a period of one year using a fingerprint method, Single Strand Conformation Polymorphism (SSCP), and the ITS-DNA sequencing of cultivable fungi, showed that shifts occurred in the fungal communities colonizing the healthy wood tissues. However, whatever the sampling time, spring, summer, autumn or winter, the fungi colonizing the healthy tissues of asymptomatic or symptomatic plants were not significantly different. Forty-eight genera were isolated, with species of Hypocreaceae and Botryosphaeriaceae being the most abundant species. Diverse fungal assemblages, made up of potentially plant-pathogenic and -protective fungi, colonized these non-necrotic tissues. Some fungi, possibly involved in GTD, inhabited the non-necrotic wood of young plants, but no increase in necrosis areas was observed over the one-year period.
Databáze: OpenAIRE
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