Effect of translocator protein (18 kDa)-ligand binding on neurotransmitter-induced salivary secretion in rat submandibular glands
| Autor: | Jean Jacques Lacapère, Alejandro Luis Arregger, Mariano A. Ostuni, Omar R. Tumilasci, Vassilios Papadopoulos, Liliana Noemí Contreras, Gabriel Péranzi, Estela M. del Luján Cardoso |
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| Rok vydání: | 2008 |
| Předmět: |
Male
Saliva PK-11195 Submandibular Gland PK 11195 translocator protein (TSPO) Biology Ciencias Biológicas chemistry.chemical_compound Biología Celular Microbiología medicine Translocator protein Animals Salivary Ducts Secretion Rats Wistar Inner mitochondrial membrane Receptor Diazepam Binding Inhibitor Neurotransmitter Agents Mucous Membrane salivary secretion Biological Transport Cell Biology General Medicine Isoquinolines Receptors GABA-A peripheral benzodiazepine receptor (PBR) Ligand (biochemistry) Molecular biology Submandibular gland Rats mitochondria medicine.anatomical_structure Biochemistry chemistry submandibular gland Mitochondrial Membranes biology.protein Carrier Proteins Salivation CIENCIAS NATURALES Y EXACTAS Protein Binding |
| Zdroj: | Biology of the Cell. 100:427-439 |
| ISSN: | 0248-4900 |
| Popis: | Background information. TSPO (translocator protein), previously known as PBR (peripheral-type benzodiazepine receptor), is a ubiquitous 18 kDa transmembrane protein that participates in diverse cell functions. High-affinity TSPO ligands are best known for their ability to stimulate cholesterol transport in organs synthesizing steroids and bile salts, although they modulate other physiological functions, including cell proliferation, apoptosis and calcium-dependent transepithelial ion secretion. In present study, we investigated the localization and function of TSPO in salivary glands. Results. Immunohistochemical analysis of TSPO in rat salivary glands revealed that TSPO and its endogenous ligand, DBI (diazepam-binding inhibitor), were present in duct and mucous acinar cells. TSPO was localized to the mitochondria of these cells, whereas DBI was cytosolic. As expected, mitochondrial membrane preparations, which were enriched in TSPO, exhibited a high affinity for the TSPO drug ligand, 3H-labelled PK 11195, as shown by Bmax and Kd values of 10.0+− 0.5 pmol/mg and 4.0+− 1.0 nM respectively. Intravenous perfusion of PK 11195 increased the salivary flow rate that was induced by muscarinic and α-adrenergic agonists, whereas it had no effect when administered alone. Addition of PK 11195 also increased the K+, Na+, Cl− and protein content of saliva, indicating that this ligand modulated secretion by acini and duct cells. Conclusions. High-affinity ligand binding to mitochondrial TSPO modulates neurotransmitter-induced salivary secretion by duct and mucous acinar cells of rat submandibular glands. Fil: Ostuni, Mariano. Université Paris Diderot - Paris 7; Francia Fil: Tumilasci, Omar Rene. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina Fil: Péranzi, Gabriel. Université Paris Diderot - Paris 7; Francia Fil: Cardoso, Estela M. del Luján. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina Fil: Contreras, Liliana Noemí. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina Fil: Arregger, Alejandro Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Médicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Médicas; Argentina Fil: Papadopoulos, Vassilios. McGill University; Canadá Fil: Lacapere, Jean Jacques. Université Paris Diderot - Paris 7; Francia |
| Databáze: | OpenAIRE |
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