Mediterranean diet supplemented with coenzyme Q10 induces postprandial changes in p53 in response to oxidative DNA damage in elderly subjects
| Autor: | Javier Delgado-Lista, Francisco Pérez-Jiménez, Pablo Perez-Martinez, Jose Lopez-Miranda, Fernando Rodriguez-Cantalejo, Jose M. Villalba-Montoro, Cristina Cruz-Teno, Justo P. Castaño, Antonio Camargo, Francisco Fuentes, Mónica Santos-González, Francisco M. Gutierrez-Mariscal, Elena M. Yubero-Serrano, Nieves Delgado-Casado |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Aging medicine.medical_specialty Antioxidant Mediterranean diet Ubiquinone DNA damage medicine.medical_treatment Blotting Western Oxidative phosphorylation Biology Diet Mediterranean Real-Time Polymerase Chain Reaction medicine.disease_cause Article chemistry.chemical_compound Internal medicine medicine Humans Aged Coenzyme Q10 Cross-Over Studies Dose-Response Relationship Drug food and beverages Vitamins General Medicine Genes p53 Postprandial Period Oxidative Stress Dose–response relationship Postprandial Endocrinology Gene Expression Regulation chemistry Dietary Supplements Female Hydroxymethylglutaryl-CoA Reductase Inhibitors Geriatrics and Gerontology Oxidation-Reduction Oxidative stress DNA Damage Follow-Up Studies |
| Zdroj: | AGE. 34:389-403 |
| ISSN: | 1574-4647 0161-9152 |
| DOI: | 10.1007/s11357-011-9229-1 |
| Popis: | Coenzyme Q10 (CoQ) is a powerful antioxidant that reduces oxidative stress. We explored whether the quality of dietary fat alters postprandial oxidative DNA damage and whether supplementation with CoQ improves antioxidant capacity by modifying the activation/stabilization of p53 in elderly subjects. In this crossover study, 20 subjects were randomly assigned to receive three isocaloric diets during 4 weeks each: (1) Mediterranean diet (Med diet), (2) Mediterranean diet supplemented with CoQ (Med+CoQ diet), and (3) saturated fatty acid-rich diet (SFA diet). Levels of mRNAs were determined for p53, p21, p53R2, and mdm2. Protein levels of p53, phosphorylated p53 (Ser20), and monoubiquitinated p53 were also measured, both in cytoplasm and nucleus. The extent of DNA damage was measured as plasma 8-OHdG. SFA diet displayed higher postprandial 8-OHdG concentrations, p53 mRNA and monoubiquitinated p53, and lower postprandial Mdm2 mRNA levels compared with Med and Med+CoQ diets (p |
| Databáze: | OpenAIRE |
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