Immunohistochemical demonstration of inter-?-trypsin inhibitor light chain (bikunin) in human mast cells
| Autor: | Tatsutoshi Nakahata, Etsuo Yoshida, Hiroshi Itoh, Hisamitsu Ide, Takahiko Kobayashi, Masaki Tomita, Yukio Osada, Haruhiko Maruyama, Yukifumi Nawa |
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| Rok vydání: | 1999 |
| Předmět: |
Histology
Connective tissue Biology Immunoglobulin light chain Pathology and Forensic Medicine Antibody Specificity Alpha-Globulins Intestine Small medicine Animals Humans Lymphocytes Mast Cells RNA Messenger Intestinal Mucosa Cells Cultured Connective Tissue Cells Glycoproteins Messenger RNA Membrane Glycoproteins Cell Biology Mast cell Immunohistochemistry Molecular medicine Molecular biology Small intestine Rats medicine.anatomical_structure Gene Expression Regulation Biochemistry Organ Specificity biology.protein Trypsin Inhibitor Kunitz Soybean Antibody |
| Zdroj: | Cell and Tissue Research. 297:149-154 |
| ISSN: | 1432-0878 0302-766X |
| DOI: | 10.1007/s004410051342 |
| Popis: | We recently reported that the rat mast cell proteinase inhibitor trypstatin is genetically identical with the second half of inter-alpha-trypsin inhibitor light chain (ITI-LC), also known as bikunin or urinary trypsin inhibitor (UTI). In this study, therefore, immunoreactivities of mast cells of various human tissues were examined with three antibodies, anti-human ITI-LC, anti-ITI, which recognizes mainly heavy chains or the sugar moiety of ITI, and anti-alpha 1-microglobulin (alpha1mG). ITI-LC immunoreactivity was strongly found in mast cells in the connective tissues of various organs except for those of the propria mucosae of small intestine. Neither anti-ITI antibody nor anti-alpha1mG antibody reacted with mast cells in various tissues. By reverse transcription-polymerase chain reaction (RT-PCR) analysis, alpha1mG/ITI-LC mRNA was not detected in the skin and tongue, and only weakly in small intestine, although ITI-LC immunoreactivity was strongly detected in these tissues. Furthermore, the mRNA was not expressed in cultured human mast cells. These results suggest that ITI-LC protein is stored in the granules of human connective tissue mast cells, though is not produced by them. |
| Databáze: | OpenAIRE |
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