HPTLC-densitometry quantification of fructooligosaccharides from inulin hydrolysate
| Autor: | Dhandeep Singh, Taranjeet Singh, Ram Sarup Singh, John F. Kennedy |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Detection limit
0303 health sciences Reproducibility Chromatography Chemistry Inulin Relative standard deviation Oligosaccharides 02 engineering and technology General Medicine Repeatability 021001 nanoscience & nanotechnology Biochemistry Hydrolysate 03 medical and health sciences Chromatographic separation chemistry.chemical_compound Structural Biology 0210 nano-technology Densitometry Molecular Biology 030304 developmental biology |
| Zdroj: | International Journal of Biological Macromolecules. 177:221-228 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2021.02.116 |
| Popis: | The objective of present research was to develop an easy, precise and accurate HPTLC densitometry method for quantification of fructooligosaccharides (FOSs) from inulin hydrolysate. The chromatographic separation of FOSs was performed on pre-coated silica gel (60, F254) TLC plates using a mobile phase (butanol:ethanol:water, 60:24:16), and densitometry evaluation of FOSs was performed at A500. Both kestose and nystose were successfully resolved with Rf value of 0.43 and 0.34, respectively. The accuracy, reliability and reproducibility of developed method was assessed by percent relative standard deviation of kestose and nystose for instrument precision (1.43% and 1.50%), repeatability (1.48% and 1.56%), intra-day precision (1.60% and 1.63%), inter-day precision (1.62% and 1.66%), limit of detection (4.58 ng/spot and 4.58 ng/spot), limit of quantification (13.87 ng/spot and 13.89 ng/spot) and recovery (98.81% and 98.69%). Moreover, overlapping spectra of test sample with standard confirms the specificity of developed method, which was validated as per ICH guidelines. |
| Databáze: | OpenAIRE |
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