Human T-cell stimulation, molecular characterization and in situ mRNA localization of a Brugia malayi recombinant antigen
| Autor: | Rocky S. Tuan, Prema Arasu, Thomas B. Nutman, Cathy Steel, Francine B. Perler, Mary M. Mulligan, David Abraham |
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| Rok vydání: | 1989 |
| Předmět: |
Adult
Male T cell T-Lymphocytes Molecular Sequence Data Clone (cell biology) Lymphocyte Activation Brugia malayi Immune system Antigen medicine Brugia Animals Humans Amino Acid Sequence RNA Messenger Cloning Molecular Molecular Biology Antiserum biology Base Sequence Genes Immunoglobulin Nucleic Acid Hybridization DNA biology.organism_classification Molecular biology Fusion protein medicine.anatomical_structure Antigens Helminth biology.protein Parasitology Female Antibody |
| Zdroj: | Molecular and biochemical parasitology. 36(3) |
| ISSN: | 0166-6851 |
| Popis: | Cellular immune responses play a major role in lymphatic filarial infections. To further our understanding of the host-parasite interaction, we investigated T-cell stimulation by purified filarial recombinant antigens in peripheral blood mononuclear cells derived from filarial-infected individuals. One of a subset of cloned Brugia malayi antigens involved in the humoral immune response to filarial infection was found to be a T-cell-stimulating antigen. The fusion protein encoded by clone lambda Bm19 induced proliferation of human T cells in a parasite-specific, antigen dose-dependent manner. The deduced amino acid sequence from this cloned region revealed 4 predicted T-cell recognition sites. The lambda Bm19 DNA sequence hybridizes to a 3-kb transcript, and in situ mRNA hybridization analyses of the adult female worm demonstrated that this gene is expressed in developing uterine microfilariae. The native parasite protein is present in several developmental stages since clone lambda Bm19 was initially identified with antiserum directed against the infective larval stage; this protein is therefore a potential target for the host's immune system. |
| Databáze: | OpenAIRE |
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