Molecular evidence of undescribed Ceratonova sp. (Cnidaria: Myxosporea) in the freshwater polychaete, Manayunkia speciosa , from western Lake Erie
| Autor: | Ann M. Thompson, David M. Malakauskas, R. Benjamin Snipes, Don W. Schloesser |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Michigan Parasitic Diseases Animal Genes Protozoan Zoology Polymerase Chain Reaction 010603 evolutionary biology 01 natural sciences Myxosporea 03 medical and health sciences Ceratomyxa shasta Phylogenetics Animals Parasite hosting Myxozoa Phylogeny Ecology Evolution Behavior and Systematics biology Phylogenetic tree Ecology Polychaeta Sequence Analysis DNA Interspecific competition Ribosomal RNA biology.organism_classification Lakes 030104 developmental biology |
| Zdroj: | Journal of Invertebrate Pathology. 137:49-53 |
| ISSN: | 0022-2011 |
| DOI: | 10.1016/j.jip.2016.05.001 |
| Popis: | We used PCR to screen pooled individuals of Manayunkia speciosa from western Lake Erie, Michigan, USA for myxosporean parasites. Amplicons from positive PCRs were sequenced and showed a Ceratonova species in an estimated 1.1% (95% CI=0.46%, 1.8%) of M. speciosa individuals. We sequenced 18S, ITS1, 5.8S, ITS2 and most of the 28S rDNA regions of this Ceratonova sp., and part of the protein-coding EF2 gene. Phylogenetic analyses of ribosomal and EF2 sequences showed the Lake Erie Ceratonova sp. is most similar to, but genetically distinct from, Ceratonova shasta. Marked interspecific polymorphism in all genes examined, including the ITS barcoding genes, along with geographic location suggests this is an undescribed Ceratonova species. COI sequences showed M. speciosa individuals in Michigan and California are the same species. These findings represent a third parasite in the genus Ceratonova potentially hosted by M. speciosa. |
| Databáze: | OpenAIRE |
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