MAGE-A4, a germ cell specific marker, is expressed differentially in testicular tumors
| Autor: | Michel Samson, Olivier De Backer, Giulio C. Spagnoli, Florence Aubry, Ewa Rajpert-De Meyts, Bernard Jégou, Anne-Pascale Satie, Patrick Chomez, Nathalie Rioux-Leclercq |
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| Rok vydání: | 2001 |
| Předmět: |
Male
endocrine system Cancer Research medicine.medical_specialty Biology Testicle Testicular Neoplasms Antigens Neoplasm Internal medicine Testis medicine Biomarkers Tumor Humans Gonads Germ cell neoplasm Cancer Seminoma medicine.disease Prognosis Embryonic stem cell Immunohistochemistry Neoplasm Proteins medicine.anatomical_structure Endocrinology Oncology Sertoli Cell Tumor Cancer research Germ cell tumors Germ cell |
| Zdroj: | Cancer. 92(11) |
| ISSN: | 0008-543X |
| Popis: | BACKGROUND Testicular germ cell tumors are the most common malignancy in young males, and the frequency of these tumors has risen dramatically over the last century. Because it is known that the MAGE genes are expressed in a wide variety of tumors but are expressed only in the mitotic spermatogonia (germ cells) and in the primary spermatocytes in the normal testis, the authors screened the expression of MAGE-A4 in a panel of testicular germ cell tumors. METHODS Monoclonal antibody 57B raised against MAGE-A4 was tested immunohistochemically on 12 classical seminomas, 5 anaplastic seminomas, 10 various specimens of nonseminomatous germ cell tumors (NSGCTs), 2 combined tumors containing seminoma components, 1 Sertoli cell tumor, 2 Leydig cell tumors, and 15 carcinomas in situ (CIS). In addition, monoclonal antibody 57B was tested on embryonic gonad (age 8 weeks) and fetal gonads (ages 15 weeks, 17 weeks, and 28 weeks). RESULTS Classical seminomas uniformly and specifically expressed MAGE-A4 compared with anaplastic seminomas and NSGCTs, which were negative for this antigen. Specific expression of MAGE-A4 also was seen in subpopulations of CIS cells, providing additional evidence for heterogeneity of the phenotype of these cells, in which it is believed that differentiation and proliferation generate seminomas and NSGCTs. Finally, MAGE-A4 was expressed in the fetal precursors of the stem germ cells from 17 weeks of gestation onward, in accordance the fact that CIS can arise from prespermatogonia in the fetus. CONCLUSIONS MAGE-A4 can be considered a potential specific marker for normal premeiotic germ cells and germ cell tumors and can be used to characterize classical seminomas. Cancer 2001;92:2778–85. © 2001 American Cancer Society. |
| Databáze: | OpenAIRE |
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