Molecular cytogenetic aberrations in Tunisian patients with multiple myeloma identified by cIg-FISH in fixed bone marrow cells
| Autor: | Hervé Avet-Loiseau, A. Khlif, Ali Saad, Abir Gmidène, M Elloumi, H Sennana, B Meddeb, I Ben Abdallah |
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| Rok vydání: | 2011 |
| Předmět: |
Adult
Male Tunisia Chromosomal translocation Bone Marrow Cells Biology Immunoglobulin light chain Translocation Genetic Immunolabeling Genetics medicine Humans Child Molecular Biology Genetics (clinical) Multiple myeloma In Situ Hybridization Fluorescence Aged Chromosome Aberrations Chromosomes Human Pair 14 Chromosomes Human Pair 13 Chromosome Karyotype Middle Aged medicine.disease Molecular biology medicine.anatomical_structure Karyotyping Immunology Cytogenetic Analysis Immunoglobulin heavy chain Female Bone marrow Chromosome Deletion Immunoglobulin Heavy Chains Multiple Myeloma |
| Zdroj: | Cytogenetic and genome research. 136(1) |
| ISSN: | 1424-859X |
| Popis: | Cytogenetic studies in multiple myeloma (MM) are hampered by the hypo-proliferative nature of plasma cells. In order to circumvent this problem, we have used a combination of immunolabeling of cytoplasmic Ig light chains (λ or ĸ) and FISH (cIg-FISH), which allowed a comprehensive detection of the most common and/or recurrent molecular cytogenetic aberrations on fixed bone marrow cells of 70 Tunisian patients. Translocations involving the chromosome 14q32 region were observed in 32 cases (45.7%), including 18 cases with a t(11;14), 8 cases with a t(4;14), and 2 cases with a t(14;16). Deletions of the 13q14 region (D13S319/RB1) were detected in 18.6%, and deletions of the 17p13 region (TP53) in 5.7% of the cases, respectively. Of all patients with a D13S319/RB1 deletion, 61.5% also carried a 14q32 translocation, whereas TP53 deletions were associated with a t(11;14) in 2 cases (50%) and a D13S319 deletion in 1 case (25%). Our results suggest that there is a correlation between the presence of 14q32 translocations and chromosome 13q14 deletions in MM patients and that cIg-FISH is more sensitive as compared to conventional karyotyping in detecting molecular cytogenetic abnormalities in this disease. |
| Databáze: | OpenAIRE |
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