The Role of Dorsal Raphe Serotonin Neurons in the Balance between Reward and Aversion

Autor: Yuma Nagai, Akihiro Yamanaka, Masashi Koda, Takayuki Nakagawa, Hisashi Shirakawa, Kaito Takayama, Shuji Kaneko, Chihiro Andoh, Kazuki Nagayasu, Naoya Nishitani
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0301 basic medicine
Male
Stimulation
Tryptophan Hydroxylase
Nucleus Accumbens
lcsh:Chemistry
Mice
0302 clinical medicine
Promoter Regions
Genetic
lcsh:QH301-705.5
Spectroscopy
reward
Neurons
TPH2
General Medicine
Dependovirus
Computer Science Applications
serotonin
Ventral tegmental area
medicine.anatomical_structure
Enhancer Elements
Genetic
psychological phenomena and processes
Dorsal Raphe Nucleus
Basal Forebrain
Genetic Vectors
Mice
Transgenic
Biology
Optogenetics
Catalysis
Article
Inorganic Chemistry
03 medical and health sciences
Dorsal raphe nucleus
medicine
Animals
Physical and Theoretical Chemistry
optogenetics
Molecular Biology
Organic Chemistry
Central Amygdaloid Nucleus
Ventral Tegmental Area
Tryptophan hydroxylase
Conditioned place preference
Mice
Inbred C57BL
030104 developmental biology
nervous system
lcsh:Biology (General)
lcsh:QD1-999
Hypothalamic Area
Lateral
Behavior Rating Scale
Serotonin
Neuroscience
030217 neurology & neurosurgery
Zdroj: International Journal of Molecular Sciences, Vol 21, Iss 6, p 2160 (2020)
International Journal of Molecular Sciences
Volume 21
Issue 6
ISSN: 1422-0067
Popis: Background: Reward processing is fundamental for animals to survive and reproduce. Many studies have shown the importance of dorsal raphe nucleus (DRN) serotonin (5-HT) neurons in this process, but the strongly correlative link between the activity of DRN 5-HT neurons and rewarding/aversive potency is under debate. Our primary objective was to reveal this link using two different strategies to transduce DRN 5-HT neurons. Methods: For transduction of 5-HT neurons in wildtype mice, adeno-associated virus (AAV) bearing the mouse tryptophan hydroxylase 2 (TPH2) gene promoter was used. For transduction in Tph2-tTA transgenic mice, AAVs bearing the tTA-dependent TetO enhancer were used. To manipulate the activity of 5-HT neurons, optogenetic actuators (CheRiff, eArchT) were expressed by AAVs. For measurement of rewarding/aversive potency, we performed a nose-poke self-stimulation test and conditioned place preference (CPP) test. Results: We found that stimulation of DRN 5-HT neurons and their projections to the ventral tegmental area (VTA) increased the number of nose-pokes in self-stimulation test and CPP scores in both targeting methods. Concomitantly, CPP scores were decreased by inhibition of DRN 5-HT neurons and their projections to VTA. Conclusion: Our findings indicate that the activity of DRN 5-HT neurons projecting to the VTA is a key modulator of balance between reward and aversion.
Databáze: OpenAIRE
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