Gene expression profiles in pregnant rats treated with T-2 toxin
| Autor: | Hiroyuki Nakayama, Takashi Yamoto, Naoki Kiyosawa, Kunio Doi, Koji Uetsuka, Munehiro Teranishi, Kyoko Sakuma, Shinya Sehata, Kazumi Ito |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
medicine.medical_specialty
Microarray Placenta Administration Oral Gene Expression Apoptosis Biology Toxicology medicine.disease_cause Pathology and Forensic Medicine Cytochrome P-450 Enzyme System Pregnancy Internal medicine Gene expression medicine Animals RNA Messenger Rats Wistar DNA Primers Oligonucleotide Array Sequence Analysis Fetus Reverse Transcriptase Polymerase Chain Reaction Microarray analysis techniques Gene Expression Profiling Cell Biology General Medicine Rats Oxidative Stress T-2 Toxin Endocrinology medicine.anatomical_structure Liver Toxicity Female Oxidative stress |
| Zdroj: | Experimental and Toxicologic Pathology. 55:357-366 |
| ISSN: | 0940-2993 |
| DOI: | 10.1078/0940-2993-00342 |
| Popis: | Summary Pregnant rats on day 13 of gestation were treated orally with T-2 toxin at a single dose of 2 mg/kg and sacrificed at 24 hours after treatment. Histopathologically, apoptosis was increased in the liver, placenta and fetal liver. Microarray analysis was performed to examine the gene expression in the liver, placenta, and fetal liver. The results of microarray analysis showed that the changes in the expression of apoptosis genes, metabolic enzymes and oxidative stress-related genes were detected in these tissues. Suppression of phase I and II enzymes-related genes expression in the liver, and suppression of phase II enzymes-related genes expression in the placenta and fetal liver were observed. Semiquantitive RT-PCR analysis also showed the same results as those of microarray analysis. From the results of microarray analysis and histopathological examination, T-2 toxin seems to induce oxidative stress in these tissues, following the changes in metabolism-related genes expression. These changes may alter the intracellular environments resulting in the induction of apoptosis. Further studies on the gene expression profiles at the earlier time point are necessary to clarify the detailed mechanisms of T-2 toxin-induced toxicity in pregnant rats. |
| Databáze: | OpenAIRE |
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