Comparison of yeast mitochondrial Phe-tRNA synthetase subunits to their cytoplasmic counterparts: Isolation and determination of amino acid compositions
| Autor: | A.J.C. Stahl, Martin Diatewa, Yves Boulanger |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
chemistry.chemical_classification
Cytoplasm Nuclear gene Macromolecular Substances Biophysics Saccharomyces cerevisiae Cell Biology Alkylation Biology Biochemistry Molecular biology Yeast Mitochondria Amino acid Amino Acyl-tRNA Synthetases Enzyme chemistry Transfer RNA β subunit Phenylalanine-tRNA Ligase Amino Acids Molecular Biology |
| Zdroj: | Biochemical and Biophysical Research Communications. 106:520-525 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(82)91141-x |
| Popis: | The α and β subunits of yeast mitochondrial Phe-tRNA synthetase are separated and isolated by means of chromatography on DEAE-cellulose, after enzyme alkylation with iodoacetate. The comparison of amino acid compositions of yeast mitochondrial and cytoplasmic native Phe-tRNA synthetases and their components shows significant differences. Results indicate that the two enzymes are coded for by different nuclear genes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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