Prevalence of occult hepatitis B in a population from the Brazilian Amazon region

Autor: Andrea Marinho da Silva, Anna Rafaella Ferreira Baraúna, Carla de Castro Sant’ Anna, Roseane Gomes de Oliveira, Evonnildo Costa Gonçalvez, Patrícia Ferreira, Marcella Kelly Costa de Almeida, Carolina de Souza Pereira, Luisa Caricio Martins
Rok vydání: 2017
Předmět:
0301 basic medicine
Male
Hepatite B / epidemiologia
HBsAg
Genotyping Techniques
T?cnicas de Genotipagem / met?dos
medicine.disease_cause
Serology
0302 clinical medicine
Genotype
Mutag?nese / gen?tica
Prevalence
Aged
80 and over
education.field_of_study
virus diseases
Hepatitis B
Middle Aged
Infectious Diseases
030211 gastroenterology & hepatology
Female
geographic locations
Brazil
Adult
Hepatitis B virus
Adolescent
Population
Biology
03 medical and health sciences
Young Adult
Virology
medicine
Humans
Hepatitis B Antibodies
education
Genotyping
V?rus da Hepatite B
Aged
Hepatitis B Surface Antigens
Molecular epidemiology
Sequence Analysis
DNA
medicine.disease
digestive system diseases
030104 developmental biology
Cross-Sectional Studies
Amino Acid Substitution
DNA
Viral
Hepatite B / diagn?stico
Zdroj: Repositório Digital do Instituto Evandro Chagas (Patuá)
Instituto Evandro Chagas (IEC)
instacron:IEC
ISSN: 1096-9071
Popis: Funda??o Amaz?nia Paraense de Amparo ? Pesquisa (Brazil) - FAPESPA, Grant number: 759557/2011 Universidade Federal do Par?. N?cleo de Medicina Tropical. Laborat?rio de Patologia de Doen?as Tropicais. Bel?m, PA, Brazil. Universidade Federal do Par?. N?cleo de Medicina Tropical. Laborat?rio de Patologia de Doen?as Tropicais. Bel?m, PA, Brazil. Universidade Federal do Par?. N?cleo de Medicina Tropical. Laborat?rio de Patologia de Doen?as Tropicais. Bel?m, PA, Brazil. Universidade Federal do Par?. N?cleo de Medicina Tropical. Laborat?rio de Patologia de Doen?as Tropicais. Bel?m, PA, Brazil. Universidade Federal do Par?. N?cleo de Medicina Tropical. Laborat?rio de Patologia de Doen?as Tropicais. Bel?m, PA, Brazil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Laborat?rio de Gen?mica. Ananindeua, PA, Brasil. Universidade Federal do Par?. N?cleo de Medicina Tropical. Laborat?rio de Patologia de Doen?as Tropicais. Bel?m, PA, Brazil. Universidade Federal do Par?. N?cleo de Medicina Tropical. Laborat?rio de Patologia de Doen?as Tropicais. Bel?m, PA, Brazil. Universidade Federal do Par?. N?cleo de Medicina Tropical. Laborat?rio de Patologia de Doen?as Tropicais. Bel?m, PA, Brazil. In the present study, we evaluated the prevalence of occult hepatitis B (OBI) in a population from the Brazilian Amazon region, identify circulating genotypes, and mutations in the S gene. One hundred eighty-one patients with negative serology for HBsAg and anti-HBs and positive serology for anti-HBc participated in the study. Detection of viral DNA, genotyping by sequencing, and analysis of nucleotide sequences to detect possible mutations were performed. HBV DNA was detected in 14.36% of the patients. Genotyping revealed genotype A in 88.46% of HBV DNA-positive subjects, with subgenotype A1 being the most prevalent (78.26%) followed by subgenotype A2 (21.74%). Genotype F was detected in 11.54% (all of them subgenotype F2). Amino acid substitutions were observed in the amplified S gene in individuals with OBI compared to HBsAg-positive individuals (evident infection). In conclusion, the results show a high prevalence of OBI in the population studied, with a pattern of genotypes A and F that circulate in the Brazilian Amazon region. Amino acid substitutions were detected in part of the S gene in patients with OBI. Further studies on the molecular epidemiology of HBV in this region are important to identify patients considered healthy but who are potential transmitters of the disease.
Databáze: OpenAIRE
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