Ultrasensitive Impedimetric Biosensor Fabricated by a New Immobilisation Technique for Parathyroid Hormone
| Autor: | Hakkı Mevlüt Özcan, Mustafa Kemal Sezgintürk, Cansu Çakar, Engin Asav, Tuba Aydin, Ayten Sagiroglu, Kübra Yildiz |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Dendrimers
Parathyroid hormone Bioengineering Biosensing Techniques macromolecular substances Applied Microbiology and Biotechnology Biochemistry chemistry.chemical_compound medicine Humans Molecular Biology Detection limit Hyperparathyroidism Chromatography Chemistry technology industry and agriculture Reproducibility of Results Electrochemical Techniques General Medicine 6-mercaptohexanol medicine.disease Dielectric spectroscopy Immobilized Proteins Linear range Dielectric Spectroscopy Calibration Microscopy Electron Scanning Glutaraldehyde Cyclic voltammetry Biosensor Electrochemical impedance spectroscopy hormones hormone substitutes and hormone antagonists Biotechnology Epiclorhidrina |
| Popis: | This paper presents a novel ultrasensitive and rapid impedimetric biosensor with new immobilisation materials for parathyroid hormone (PTH) with the aim to determine the PTH level in serum for the diagnosis and monitoring of parathyroid diseases such as hyperparathyroidism, adenoma, and thyroid cancer. The interaction between PTH and the biosensor was investigated with an electrochemical method. The biosensor was based on the gold electrode modified by mercaptohexanol (6-MHL). Anti-parathyroid hormone (anti-PTH) was covalently immobilised onto a self-assembled monolayer (SAM) by using epiclorhidrina (EPI) with ethanolamine (EA). The EPI-EA interaction represents the first use of these for the construction of biosensors in published reports. The immobilisation of the anti-PTH was monitored by electrochemical impedance spectroscopy, cyclic voltammetry and scanning electron microscopy (SEM) techniques. After the optimisation studies of immobilisation materials such as 6-MHL, EPI, EA and glutaraldehyde, linearity, repeatability and sensitivity of biosensor were evaluated as the performance of biosensor. PTH was detected within a linear range of 0.1-0.6 pg/ml, and the detection limit was 0.1 fg/ml. The specificity of the biosensor was also investigated. Finally, the described biosensor was used to detect the PTH levels in artificial serum samples. Trakya University Scientific Research FoundationTrakya University [TUBAP-2001/195]; TUBITAK (The Scientific and Technological Research Council of Turkey)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [113 Z 678] Support from the Trakya University Scientific Research Foundation (project number: TUBAP-2001/195) and TUBITAK (The Scientific and Technological Research Council of Turkey; project number: 113 Z 678) are greatly acknowledged. |
| Databáze: | OpenAIRE |
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