Effects of docosahexaenoic acid on in vitro amyloid beta peptide 25–35 fibrillation
| Autor: | Masanori Katakura, Yoko Tanabe, Shuji Gamoh, Toshio Shimada, Hossain Md Shahdat, Koji Miwa, Osamu Shido, Michio Hashimoto |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Amyloid
Docosahexaenoic Acids Amyloid beta Peptide In Vitro Techniques chemistry.chemical_compound mental disorders medicine Humans Benzothiazoles Molecular Biology Fluorescent Dyes chemistry.chemical_classification Fibrillation Amyloid beta-Peptides Microscopy Confocal Dose-Response Relationship Drug biology Neurotoxicity P3 peptide Neurofibrillary Tangles Cell Biology medicine.disease Peptide Fragments Thiazoles chemistry Biochemistry Docosahexaenoic acid biology.protein Thioflavin medicine.symptom |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids. 1791(4):289-296 |
| ISSN: | 1388-1981 |
| Popis: | Amyloid beta peptide(25-35) (Abeta(25-35)) encompasses one of the neurotoxic domains of full length Abeta(1-40/42), the major proteinaceous component of amyloid deposits in Alzheimer's disease (AD). We investigated the effect of docosahexaenoic acid (DHA, 22:6, n-3), an essential brain polyunsaturated fatty acid, on the in vitro fibrillation of Abeta(25-35) and found that it significantly reduced the degree of fibrillation, as shown by a decrease in the intensity of both the thioflavin T and green fluorescence in confocal microscopy. Transmission electron microscopy revealed that DHA-incubated samples were virtually devoid of structured fibrils but had an amorphous-like consistency, whereas the controls contained structured fibers of various widths and lengths. The in vitro fibrillation of Abeta(25-35) appeared to be pH-dependent, with the strongest effect seen at pH 5.0. DHA inhibited fibrillation at all pHs, with the strongest effect at pH 7.4. It also significantly decreased the levels of Abeta(25-35) oligomers. Nonreductive gradient gel electrophoresis revealed that the molecular size of the oligomers of Abeta(25-35) was 10 kDa (equivalent to decamers of Abeta(25-35)) and that DHA dose-dependently reduced these decamers. These results suggest that DHA decreases the in vitro fibrillation of Abeta(25-35) by inhibiting the oligomeric amyloid species and, therefore, Abeta(25-35)-related neurotoxicity or behavioral impairment could be restrained by DHA. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect