Allogeneic vaccination for renal cell carcinoma: development and monitoring
| Autor: | Dolores J. Schendel, Birgit Stadlbauer, B Dörken, Gerald Willimsky, Ralph Oberneder, Bernhard Frankenberger, Alfons Hofstetter, Heike Pohla, Antonio Pezzutto, Thomas Blankenstein |
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| Rok vydání: | 2000 |
| Předmět: |
Receptors
Antigen T-Cell Priming (immunology) Human leukocyte antigen In Vitro Techniques Major histocompatibility complex Cancer Vaccines Epitope Antigen Monitoring Immunologic Tumor Cells Cultured Humans Transplantation Homologous Medicine Cytotoxic T cell Allogeneic Tumor Cell Vaccine Carcinoma Renal Cell Transplantation biology Reverse Transcriptase Polymerase Chain Reaction business.industry Vaccination T-cell receptor Hematology Cytotoxicity Tests Immunologic Kidney Neoplasms Immunology biology.protein Metals Rare Earth business Neoplasm Transplantation T-Lymphocytes Cytotoxic |
| Zdroj: | Bone Marrow Transplantation. 25:S83-S87 |
| ISSN: | 1476-5365 0268-3369 |
| DOI: | 10.1038/sj.bmt.1702362 |
| Popis: | An allogeneic tumor cell vaccine should display a natural immunogenicity that allows the stimulation of tumor-reactive effector cells in patients. Furthermore, the vaccine should express antigens that are shared by many tumors to which patients are not tolerant. A variety of tumor peptides should be presented by different HLA-molecules due to limited MHC matching with recipients and last but not least, the vaccine should have a strong growth potential in vitroto allow adequate amounts of vaccine to be generated for long-term usage. In vitro and in situ studies with the renal cell carcinoma cell line RCC-26 demonstrate: (1) RCC-26 can induce complex allospecific responses through direct priming; (2) RCC-26 can not only reactivate cytotoxic T lymphocytes (CTL) of a memory phenotype but they also can induce de novo tumor-antigen associated responses in normal donors; (3) these cells present epitopes restricted by several MHC molecules, allowing the vaccination of patients matched for different HLA alleles; and (4) they stimulate HLA-A*0201-restricted T cells bearing characteristic T cell receptors (TCR). Thus, in addition to using limiting dilution killer and ELISPOT assays, molecular tracking of a tumor-specific TCR can be used to judge the development of antitumor reactivity and vaccine efficiency. Bone Marrow Transplantation (2000) 25, Suppl. 2, S83–S87. |
| Databáze: | OpenAIRE |
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