Anti–MSP-10 IgG indicates recent exposure to Plasmodium vivax infection in the Peruvian Amazon
| Autor: | Angel Rosas-Aguirre, Dionicia Gamboa, Robert H. Gilman, Carlos Fernández, Kailash P. Patra, Yossef Alnasser, Joseph M. Vinetz, Edith Arocutipa, Grace Trompeter, Edith Málaga, Maritza Calderon, Katherine Torres, Katherine Garro, Alejandro Llanos-Cuentas |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Adult Male Allergy medicine.medical_specialty Adolescent Plasmodium vivax Plasmodium falciparum Protozoan Proteins Peruvian Amazon Antigens Protozoan Serology Cohort Studies 03 medical and health sciences Young Adult 0302 clinical medicine Epidemiology Peru parasitic diseases medicine Malaria Vivax Humans Malaria Falciparum Child Anti–MSP-10 IgG biology Amazon rainforest business.industry General Medicine medicine.disease biology.organism_classification Virology purl.org/pe-repo/ocde/ford#3.02.00 [https] Titer 030104 developmental biology Infectious disease (medical specialty) 030220 oncology & carcinogenesis Child Preschool Immunoglobulin G Multivariate Analysis Female Clinical Medicine business Infection Malaria |
| Popis: | BACKGROUND. Serological tools for the accurate detection of recent malaria exposure are needed to guide and monitor malaria control efforts. IgG responses against Plasmodium vivax and P. falciparum merozoite surface protein-10 (MSP10) were measured as a potential way to identify recent malaria exposure in the Peruvian Amazon. METHODS. A field-based study included 470 participants in a longitudinal cohort who completed a comprehensive evaluation: light microscopy and PCR on enrollment, at least 1 monthly follow-up by light microscopy, a second PCR, and serum and dried blood spots for serological analysis at the end of the follow-up. IgG titers against novel mammalian cell–produced recombinant PvMSP10 and PfMSP10 were determined by ELISA. RESULTS. During the follow-up period, 205 participants were infected, including 171 with P. vivax, 26 with P. falciparum, 6 with infections by both species but at different times, and 2 with mixed infections. Exposure to P. vivax was more accurately identified when serological responses to PvMSP10 were obtained from serum (sensitivity, 58.1%; specificity, 81.8%; AUC: 0.76) than from dried blood spots (sensitivity, 35.2; specificity, 83.5%; AUC: 0.64) (PAUC < 0.001). Sensitivity was highest (serum, 82.9%; dried blood spot, 45.7%) with confirmed P. vivax infections occurring 7–30 days before sample collection; sensitivity decreased significantly in relation to time since last documented infection. PvMSP10 serological data did not show evidence of interspecies cross-reactivity. Anti-PfMSP10 responses poorly discriminated between P. falciparum–exposed and nonexposed individuals (AUC = 0.59; P > 0.05). CONCLUSION. Anti-PvMSP10 IgG indicates recent exposure to P. vivax at the population level in the Amazon region. Serum, not dried blood spots, should be used for such serological tests. FUNDING. Cooperative agreement U19AI089681 from the United States Public Health Service, NIH/ National Institute of Allergy and Infectious Diseases, as the Amazonian International Center of Excellence in Malaria Research. |
| Databáze: | OpenAIRE |
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