Characterization of a novel panel of plasma microRNAs that discriminates between Mycobacterium tuberculosis infection and healthy individuals
| Autor: | Gao-Xiang Sun, Enyu Dai, Hong Ling, Xi Chen, Di Li, Xiao-Yu He, Donghai Li, Yanhong Liu, Chen-Yu Zhang, Jin Fu, Jia-Yi Cui, Hongwei Liang, Chihao Zhao, Xin-Ling Pan, Na Jiao, Ke Zen, Chun-Lei Zhang, Fengmin Zhang |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Bacterial Diseases Physiology Molecular biology Extensively Drug-Resistant Tuberculosis lcsh:Medicine Biochemistry 0302 clinical medicine Sequencing techniques Blood plasma Tuberculosis Multidrug-Resistant Medicine and Health Sciences lcsh:Science Multidisciplinary biology Reverse Transcriptase Polymerase Chain Reaction RNA sequencing Middle Aged Body Fluids Nucleic acids Actinobacteria Infectious Diseases Blood 030220 oncology & carcinogenesis Healthy individuals Biomarker (medicine) Female Anatomy Research Article Adult Tuberculosis Adolescent Blood Plasma Mycobacterium tuberculosis 03 medical and health sciences Extraction techniques Pulmonary tuberculosis microRNA medicine Genetics Humans Non-coding RNA Tuberculosis Pulmonary Biology and life sciences Bacteria business.industry lcsh:R Organisms Extensively drug-resistant tuberculosis medicine.disease biology.organism_classification Tropical Diseases RNA extraction Gene regulation Research and analysis methods MicroRNAs 030104 developmental biology Molecular biology techniques Immunology RNA lcsh:Q Gene expression business Biomarkers Mycobacterium Tuberculosis |
| Zdroj: | PLoS ONE PLoS ONE, Vol 12, Iss 9, p e0184113 (2017) |
| ISSN: | 1932-6203 |
| Popis: | Cavities are important in clinical diagnosis of pulmonary tuberculosis (TB) infected by Mycobacterium tuberculosis. Although microRNAs (miRNAs) play a vital role in the regulation of inflammation, the relation between plasma miRNA and pulmonary tuberculosis with cavity remains unknown. In this study, plasma samples were derived from 89 cavitary pulmonary tuberculosis (CP-TB) patients, 89 non-cavitary pulmonary tuberculosis (NCP-TB) patients and 95 healthy controls. Groups were matched for age and gender. In the screening phase, Illumina high-throughput sequencing technology was employed to analyze miRNA profiles in plasma samples pooled from CP-TB patients, NCP-TB patients and healthy controls. During the training and verification phases, quantitative RT-PCR (qRT-PCR) was conducted to verify the differential expression of selected miRNAs among groups. Illumina high-throughput sequencing identified 29 differentially expressed plasma miRNAs in TB patients when compared to healthy controls. Furthermore, qRT-PCR analysis validated miR-769-5p, miR-320a and miR-22-3p as miRNAs that were differently present between TB patients and healthy controls. ROC curve analysis revealed that the potential of these 3 miRNAs to distinguish TB patients from healthy controls was high, with the area under the ROC curve (AUC) ranged from 0.692 to 0.970. Moreover, miR-320a levels were decreased in drug-resistant TB patients than pan-susceptible TB patients (AUC = 0.882). In conclusion, we identified miR-769-5p, miR-320a and miR-22-3p as potential blood-based biomarkers for TB. In addition, miR-320a may represent a biomarker for drug-resistant TB. |
| Databáze: | OpenAIRE |
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