Methods to Study Roles of β-Arrestins in the Regulation of Pancreatic β-Cell Function
| Autor: | Safia Costes, Stéphane Dalle, Gyslaine Bertrand, Magalie A. Ravier |
|---|---|
| Přispěvatelé: | Institut de Génomique Fonctionnelle (IGF), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Genotyping genetic structures Subcellular fractionation Immunocytochemistry Immunofluorescence Western blot [SDV.BC]Life Sciences [q-bio]/Cellular Biology Receptor tyrosine kinase 03 medical and health sciences 0302 clinical medicine Small interference RNA Free fatty acid receptor 1 medicine Receptor Pancreatic beta-cell beta-Arrestins medicine.diagnostic_test biology Chemistry Beta-Arrestins Pancreatic islets Cell biology Insulin receptor 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis biology.protein sense organs |
| Zdroj: | Methods in Molecular Biology Methods in Molecular Biology, 1957, springer; Humana Press, pp.345-364, 2019, Beta-arrestins, 978-1-4939-9157-0. ⟨10.1007/978-1-4939-9158-7_22⟩ Beta-Arrestins ISBN: 9781493991570 |
| Popis: | International audience; Novel findings reveal important functional roles for β-arrestin 1 and β-arrestin 2 in the regulation of insulinsecretion, β-cell survival, and β-cell mass plasticity not only by glucose but also by G-protein-coupledreceptors, such as the glucagon-like peptide-1 (GLP-1) and the pituitary adenylate cyclase-activatingpolypeptide (PACAP) receptors or GPR40, or tyrosine kinase receptors, such as the insulin receptor.Here, we describe experimental protocols to knock down β-arrestins by small interference RNA, to followsubcellular localization of β-arrestins in the cytosol and nucleus of the insulinoma INS-1E rat pancreaticβ-cell line, and to analyze β-arrestin protein expression by Western blot using INS-1E cells and isolatedmouse or human pancreatic islets. We also provide details on how to genotype β-arrestin 2 knockout(Arrb2-/-) mice and to evaluate β-arrestin-mediated roles in β-cell mass plasticity and β-cell signaling usingimmunocytochemistry on pancreatic sections or on primary dispersed β-cells from wild-type mice andArrb2-/- mice. |
| Databáze: | OpenAIRE |
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