Characterization of [3H]-valinomycin binding to red blood cell membrane
Autor: | C. R. Levine, M. Herzberg |
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Rok vydání: | 1980 |
Předmět: |
Erythrocytes
Reticulocytes Irreversible binding Biology In Vitro Techniques Valinomycin chemistry.chemical_compound Membrane Lipids Genetics medicine Animals Molecular Biology Volume concentration Binding Sites Erythrocyte Membrane Temperature Biological activity General Medicine Hydrogen-Ion Concentration Red blood cell medicine.anatomical_structure Biphasic Pattern Membrane chemistry Biochemistry Rabbits |
Zdroj: | Molecular biology reports. 6(3) |
ISSN: | 0301-4851 |
Popis: | Valinomycin was tritiated by exchange and its biological activity found to be similar to that of non-labeled drug. [3H]-valinomycin binds to red blood cell membranes following a biphasic pattern. High concentrations of the drug lead to an irreversible binding while low concentrations lead to a completely reversible binding. Maximum binding was obtained at acidic pH (pH 4.2) and physiological temperature (37 degrees C). We demonstrate that valinomycin binds strongly to the lipidic phase of the membrane. When binding to erythrocytes and reticulocytes was compared, it was found that the mature red blood cells had less binding capacity than the reticulocytes. |
Databáze: | OpenAIRE |
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