Interaction of smoothened with integrin‐linked kinase in primary cilia mediates Hedgehog signalling
| Autor: | Shoukat Dedhar, Jason E. Cain, Luciano G. Martellotto, Badia Maria Barakat, Gregory E. Hannigan, Liang Yu, Elisabetta De Luca, Die Wang, Anthony J. Sadler, D. Neil Watkins, Camden Lo, Duc Vu |
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| Rok vydání: | 2013 |
| Předmět: |
animal structures
Protein Serine-Threonine Kinases Biochemistry Receptors G-Protein-Coupled GLI2 Cerebellum Genetics Animals Hedgehog Proteins Integrin-linked kinase Cilia Hedgehog Molecular Biology biology Effector Cilium Scientific Reports Cell biology Smoothened Receptor Hedgehog signalling embryonic structures biology.protein Signal transduction Smoothened Signal Transduction |
| Zdroj: | EMBO reports. 14:945-945 |
| ISSN: | 1469-3178 1469-221X |
| DOI: | 10.1038/embor.2013.150 |
| Popis: | Here we report that ILK localizes in the mouse primary cilium, a sensory organelle required for signalling by the Hedgehog (Hh) pathway. Genetic or pharmacological inhibition of ILK blocks ciliary accumulation of the Hh pathway effector smoothened (Smo) and suppresses the induction of Gli transcription factor mRNAs by SHh. Conditional deletion of ILK or Smo also inhibits SHh-driven activation of Gli2 in the embryonic mouse cerebellum. ILK regulation of Hh signalling probably requires the physical interaction of ILK and Smo in the cilium, and we also show selective cilia-associated interaction of ILK with β-arrestin, a known mediator of Smo-dependent signalling. |
| Databáze: | OpenAIRE |
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