Immunohistochemical localization of megalin and cubilin in the human inner ear
| Autor: | Akira Ishiyama, Kumiko Hosokawa, Seiji Hosokawa, Ivan A. Lopez, Gail Ishiyama |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Endocytic cycle Reissner’s membrane urologic and male genital diseases Endolymphatic duct 0302 clinical medicine Vestibular end organs Receptors 80 and over Psychology Receptor Aged 80 and over General Neuroscience Reissner's membrane Ear Immunohistochemistry Endocytosis Cell biology Cochlea Low Density Lipoprotein Receptor-Related Protein-2 medicine.anatomical_structure Cell Surface Cognitive Sciences Female Vestibule Labyrinth Vestibule Receptors Cell Surface Biology Article 03 medical and health sciences Megalin Utricle medicine otorhinolaryngologic diseases Humans Inner ear Labyrinth Molecular Biology Aged Neurology & Neurosurgery Neurosciences Temporal Bone Epithelial Cells Inner Cubilin Human inner ear 030104 developmental biology Ear Inner Neurology (clinical) sense organs 030217 neurology & neurosurgery Developmental Biology |
| Popis: | Megalin and cubilin are endocytic receptors expressed in many absorptive polarized epithelia. These receptors have been implicated in the transport of gentamicin in the inner ear as possible contributors to ototoxic damage. Megalin and cubilin have been characterized in detail in the mouse and rat inner ear, but not in the human inner ear. In this study, megalin and cubilin were localized by immunohistochemistry using affinity-purified antibodies in formalin fixed frozen cryostat and celloidin embedded sections of the human inner ear. In the cochlea megalin and cubilin were localized in marginal cells of the stria vascularis, epithelial cells of the spiral prominence and the Reissner's membrane. In the macula utricle and cristae ampullaris, megalin and cubilin were localized in transitional and dark cells, but not in vestibular hair cells and supporting cells. In the endolymphatic duct megalin and cubilin were localized in the epithelial cells. The localization of megalin and cubilin in the human inner ear is consistent with previous reports in the inner ear of animal models and suggest that these receptors may play an important role in the inner ear endocytic transport, and maybe potential targets for prevention of ototoxic damage or the delivery of medications. |
| Databáze: | OpenAIRE |
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