Differential ontogeny of in vitro vascular responses to three categories of calcium channel antagonists in rats
| Autor: | Venkataraman Balaraman, Bruce E. Pichoff, Wayne M Ichimura, Kenneth T Nakamura, Linda K Kullama, John R. Claybaugh |
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| Rok vydání: | 1991 |
| Předmět: |
Male
medicine.medical_specialty Contraction (grammar) Endothelium Nifedipine Aorta Thoracic Isometric exercise In Vitro Techniques Diltiazem Internal medicine medicine Animals ED50 Chemistry Calcium channel Age Factors Rats Inbred Strains Calcium Channel Blockers Rats Vasodilation medicine.anatomical_structure Endocrinology Verapamil Pediatrics Perinatology and Child Health Female Calcium Channels medicine.drug |
| Zdroj: | Pediatric research. 29(3) |
| ISSN: | 0031-3998 |
| Popis: | We examined the ontogeny of relaxation responses to three categories of calcium channel antagonists, represented by verapamil, diltiazem, and nifedipine, for both potential-operated (KCl-mediated) and receptor-operated channels [norepinephrine (NE)-mediated] in rat thoracic aorta. Aortic rings from 2− to 3-d, 1-wk, and 12-wk-old Sprague Dawley rats were mounted in an organ bath, bathed in Krebs' solution, and connected to a force-displacement transducer to measure isometric tension. Endothelium intact vessels at optimal passive force were exposed to a single ED50 of isotonic KCl or NE, equilibrium contraction was measured, then vessels were washed and exposed for 30 min to 1 μM verapamil, 1 μM diltiazem, or 0.1 μM nifedipine, followed by another dose of KCl or NE. Verapamil and diltiazem demonstrated significant (p < 0.05) age-related increases in effectiveness for blocking KCl-mediated contraction [(% reduction of control contraction ± SEM) (Verapamil: 2–3 d, 67.7 ± 4.2; 1 wk, 72.5 ± 1.8; 12 wk, 89.5 ± 1.0. Diltiazem: 2–3 d, 64.6 ± 2.9; 1 wk, 73.5 ± 3.0; 12 wk, 83.1 ± 1.8]. Nifedipine was equally effective at all ages: 2–3 d, 85.6 ± 1 1.3; wk, 90.0 ± 1.6; and 12 wk, 91.3 ± 1.4. Verapamil and diltiazem also showed significant age-related increases in effectiveness for blocking NE-mediated contraction (Verapamil: 2–3 d, 6.2 ± 3.9; 1 wk, 28.0 ± 4.8; 12 wk, 44.1 ± 6.0. Diltiazem: 2–3 d, 8.0 ± 3.1; 1 wk, 20.5 ± 3.9; 12 wk, 46.5 ± 4.8). Again, nifedipine was equally effective at all ages: 2–3 d, 42.0 ± 6.8; 1 wk, 35.8 ± 3.9; and 12 wk, 37.5 ± 3.2. In summary, for the categories of calcium channel antagonists that interact at the phenylalkytomine (verapamil) and benzothiazepine (diltiazem) binding sites, there were age-related increases in effectiveness for blocking both potential-operated and receptor-operated channels. However, for nifedipine, which binds to the 1,4-dihydropyridine binding site, no maturational change was observed. These results suggest that the ontogeny of calcium channel antagonists' function may vary depending on the site of binding within the calcium channel. |
| Databáze: | OpenAIRE |
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