The Glycosylation Pathway of Eimeria tenella Is Upregulated during Gametocyte Development and May Play a Role in Oocyst Wall Formation▿
Autor: | Nicholas Smith, Iveta Slapetova, Robert A. Walker, Jan Šlapeta, Catherine M. Miller |
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Jazyk: | angličtina |
Rok vydání: | 2009 |
Předmět: |
Glycosylation
animal diseases Nitrogenous Group Transferases Molecular Sequence Data Protozoan Proteins Biology Microbiology Eimeria chemistry.chemical_compound Transcription (biology) Glucosamine Cell Wall Gene Expression Regulation Fungal Lectins parasitic diseases Gametocyte Animals Molecular Biology Gene chemistry.chemical_classification Molecular Structure Fructosephosphates Oocysts General Medicine Articles biology.organism_classification Molecular biology Cell biology carbohydrates (lipids) Enzyme chemistry lipids (amino acids peptides and proteins) Glycoprotein Chickens |
Popis: | Sexual-stage glycoproteins of Eimeria are important components of the oocyst wall, a structure that ensures the efficient transmission of these and related parasites. In this study, the primary enzyme in the glycosylation pathway of Eimeria tenella , glucosamine:fructose-6-phosphate aminotransferase (EtGFAT), has been characterized as a macrogamete-specific protein. Although the transcription of Et GFAT was observed early in macrogamete development, protein expression was restricted to mature macrogametes, prior to their conversion into unsporulated oocysts. Genes coding for three other enzymes required for N -acetylgalactosamine (GalNAc) synthesis were also transcribed during E. tenella macrogamete development. Gene transcription of the enzyme responsible for the O-linked transfer of GalNAc to proteins, EtGalNAc-T, was upregulated primarily in unsporulated oocyst stages, and accordingly, a significant increase in GalNAc levels was observed in E. tenella gametocytes and oocysts. Gam56 and Gam82, two well-characterized glycoproteins of Eimeria macrogametes and the oocyst wall, contain high levels of GalNAc and represent probable targets of GalNAc O linkage. It appears that the glycosylation pathway, specifically relating to the formation of GalNAc O links, is dramatically upregulated in E. tenella sexual stages and may play a role in directing a number of macrogamete proteins to the developing oocyst wall. |
Databáze: | OpenAIRE |
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