Changes in Levels of Seminal Nitric Oxide Synthase, Macrophage Migration Inhibitory Factor, Sperm DNA Integrity and Caspase-3 in Fertile Men after Scrotal Heat Stress
| Autor: | Lei-Guang Wang, Mei-Hua Zhang, Qiu Yi, Ai-Dong Zhang, Zhi-Da Shi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Adult
Male Hot Temperature lcsh:Medicine Semen DNA Fragmentation Biology Semen analysis Heat Stress Disorders Nitric Oxide complex mixtures Nitric oxide Andrology Semen quality chemistry.chemical_compound medicine Humans lcsh:Science Macrophage Migration-Inhibitory Factors Sperm motility Multidisciplinary medicine.diagnostic_test Sperm Count urogenital system Caspase 3 lcsh:R Sperm Spermatozoa humanities Healthy Volunteers Semen Analysis chemistry Sperm Motility DNA fragmentation lcsh:Q Macrophage migration inhibitory factor Nitric Oxide Synthase Research Article |
| Zdroj: | PLoS ONE PLoS ONE, Vol 10, Iss 10, p e0141320 (2015) |
| ISSN: | 1932-6203 |
| Popis: | Background This study observes changes in levels of seminal nitric oxide (NO), nitric oxide synthase (NOS), macrophage migration inhibitory factor (MIF), sperm DNA integrity, chromatin condensation and Caspase-3in adult healthy men after scrotal heat stress (SHS). Methods Exposure of the scrotum of 25 healthy male volunteers locally at 40–43°C SHS belt warming 40 min each day for successive 2 d per week. The course of SHS was continuously 3 months. Routine semen analysis, hypo-osmotic swelling (HOS) test, Aniline blue (AB) staining, HOS/AB and terminal deoxynucleotidyl transferase-mediated d UDP nick-end labeling (TUNEL) were carried out before, during and after SHS. Seminal NO and NOS contents were determined by nitrate reduction method. The activated Caspase-3 levels of spermatozoa and MIF in seminal plasma were measured by the enzyme-linked immunosorbent assay (ELISA) method. Statistical significance between mean values was determined using statistical ANOVA tests. Results The mean parameters of sperm concentration, motile and progressive motile sperm and normal morphological sperm were significantly decreased in groups during SHS 1, 2 and 3 months compared with those in groups of pre-SHS (P |
| Databáze: | OpenAIRE |
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