Contact hypersensitivity reaction to ovalbumin in newborn guinea pigs from maternally sensitized animals
| Autor: | Abdul Manan Bhutto, Sadao Anan, Hikotaro Yoshida, Minoru Honda, Keisuke Maeda |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Male
Injections Intradermal Ovalbumin Guinea Pigs Administration Oral Dermatology Dermatitis Contact Biochemistry Group B Antigen Pregnancy Oral administration medicine Animals Intradermal injection Molecular Biology White petrolatum Sensitization biology business.industry Passive Cutaneous Anaphylaxis Patch Tests Disease Models Animal medicine.drug_formulation_ingredient Titer medicine.anatomical_structure Animals Newborn Immunoglobulin G Immunology biology.protein Female business Immunity Maternally-Acquired Food Hypersensitivity |
| Zdroj: | Journal of Dermatological Science. 4:156-165 |
| ISSN: | 0923-1811 |
| DOI: | 10.1016/0923-1811(92)90014-3 |
| Popis: | An animal study was conducted to elucidate the role of ovalbumin (OA) in the development of eczematous lesions in intrauterine sensitized newborns. Four groups of pregnant guinea pigs were used: group A, immunized by oral administration of 1% OA in drinking water until parturition; group B, immunized by intradermal injection of OA with Freund's complete adjuvant; group C, immunized by both methods; and group D (control), not immunized. The newborn guinea pigs of each group were patch tested with 10% OA in white petrolatum. Positive reactions were seen in the newborns of groups B and C, but not in those in groups A and D. By enzyme-linked immunosorbent assay and passive cutaneous anaphylaxis, a high titre of OA-specific IgG was detected in the group B and C newborns. The number of positive patch test reactions decreased concomitantly with the decline of specific IgG. Histologically, eczematous changes were observed in the positive reaction sites. Many OA antigen-bearing Langerhans cells were found by the immuno-double labelling technique. Immuno-electron microscopic findings revealed the presence of OA antigens as well as IgG molecules on the cytoplasmic membranes of Langerhans cells. Our studies demonstrated that maternal sensitization with OA can induce an eczematous reaction in the newborns to OA patch testing under the presence of high levels of OA-specific IgG in the serum. From these findings it is suggested that IgG plays an essential role in the development of contact hypersensitivity reaction to OA. |
| Databáze: | OpenAIRE |
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