Physiological basis for isoxadifen-ethyl induction of nicosulfuron detoxification in maize hybrids
| Autor: | Renhai Wu, Lanlan Sun, Wangcang Su, Zenggui Gao, Lu Chuantao |
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| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
Acetolactate synthase activity Pyridines Enzyme Metabolism lcsh:Medicine Toxicology Pathology and Laboratory Medicine 01 natural sciences Biochemistry chemistry.chemical_compound Cytochrome P-450 Enzyme System Medicine and Health Sciences Bioassay lcsh:Science Enzyme Chemistry Oxazoles Glutathione Transferase Multidisciplinary biology Organic Compounds Chromatographic Techniques Agriculture 04 agricultural and veterinary sciences Plants Glutathione Organophosphates Chemistry Experimental Organism Systems Amino Acid Specific Chromatography Inactivation Metabolic Physical Sciences Malathion Metabolic Pathways Agrochemicals Detoxification Plant Shoots Research Article Ethers Piperonyl butoxide medicine.drug_class Research and Analysis Methods Zea mays Model Organisms Plant and Algal Models medicine Glutathione Chromatography Xenobiotic Metabolism Grasses Acetolactate synthase Herbicides Affinity Chromatography lcsh:R Organic Chemistry Organisms Chemical Compounds Biology and Life Sciences Monooxygenase Sulfonylurea Maize Enzyme Activation Acetolactate Synthase Sulfonylurea Compounds Metabolism chemistry Agronomy Seedlings 040103 agronomy & agriculture biology.protein Enzymology 0401 agriculture forestry and fisheries lcsh:Q 010606 plant biology & botany |
| Zdroj: | PLoS ONE PLoS ONE, Vol 12, Iss 3, p e0173502 (2017) |
| ISSN: | 1932-6203 |
| Popis: | Isoxadifen-ethyl can effectively alleviate nicosulfuron injury in the maize. However, the effects of safener isoxadifen-ethyl on detoxifying enzymes in maize is unknown. The individual and combined effects of the sulfonylurea herbicide nicosulfuron and the safener isoxadifen-ethyl on the growth and selected physiological processes of maize were evaluated. Bioassays showed that the EC50 values of nicosulfuron and nicosulfuron plus isoxadifen-ethyl for maize cultivar Zhengdan958 were 18.87 and 249.28 mg kg-1, respectively, and were 24.8 and 275.51 mg kg-1, respectively, for Zhenghuangnuo No. 2 cultivar. Evaluations of the target enzyme of acetolactate synthase showed that the I50 values of nicosulfuron and nicosulfuron plus isoxadifen-ethyl for the ALS of Zhengdan958 were 15.46 and 28.56 μmol L-1, respectively, and were 0.57 and 2.17 μmol L-1, respectively, for the acetolactate synthase of Zhenghuangnuo No. 2. The safener isoxadifen-ethyl significantly enhanced tolerance of maize to nicosulfuron. The enhanced tolerance of maize to nicosulfuron in the presence of the safener, coupled with the enhanced injury observed in the presence of piperonyl butoxide, 1-aminobenzotriazole, and malathion, suggested cytochrome P450 monooxygenases may be involved in metabolism of nicosulfuron. We proposed that isoxadifen-ethyl increases plant metabolism of nicosulfuron through non-P450-catalyzed routes or through P450 monooxygenases not inhibited by piperonyl butoxide, 1-aminobenzotriazole, and malathion. Isoxadifen-ethyl, at a rate of 33 mg kg-1, completely reversed the effects of all doses (37.5-300 mg kg-1) of nicosulfuron on both of the maize cultivars. When the two compounds were given simultaneously, isoxadifen-ethyl enhanced activity of glutathione S-transferases (GSTs) and acetolactate synthase activity in maize. The free acid 4,5-dihydro-5,5-diphenyl-1,2-oxazole-3-carboxylic was equally effective at inducing GSTs as the parent ester and appeared to be the active safener. GST induction in the maize Zhenghuangnuo No. 2 was faster than in Zhengdan 958. |
| Databáze: | OpenAIRE |
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