Streptozotocin toxicity in vitro depends on maturity of neurons
| Autor: | M. R. Kapkaeva, Elisaveta E. Genrikhs, Elena V. Stelmashook, Nickolay K. Isaev, D. N. Voronkov |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Neurogenesis Primary Cell Culture chemistry.chemical_element Glutamic Acid Neural Cell Adhesion Molecule L1 Calcium Toxicology Streptozocin 03 medical and health sciences 0302 clinical medicine In vivo Internal medicine Cerebellum medicine Animals Rats Wistar Cells Cultured Pharmacology Neurons Cell Death Chemistry Neurodegeneration Glutamate receptor medicine.disease Streptozotocin In vitro 030104 developmental biology Endocrinology nervous system Animals Newborn Toxicity Nerve Degeneration Sialic Acids 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Toxicology and applied pharmacology. 348 |
| ISSN: | 1096-0333 |
| Popis: | Streptozotocin (STZ) is a glucosamine-nitrosourea compound that is particularly toxic to the insulin-producing beta-cells of the pancreas in mammals; it is used for experimental simulation of sporadic Alzheimer's disease by means of intracerebroventricular administration in vivo. Here we show that the application of 3–4 mM STZ to primary culture for 48 h induces neuronal death in immature (2–3 days in vitro) cultures of rat cerebellar granule cells. Mature cultures (7–8 days in vitro) were poorly sensitive to this toxic treatment. Immature cultures demonstrated a high expression of the protein PSA-NCAM, the marker of immature neurons, and they were insensitive to the toxic effect of glutamate. In mature cultures, this protein was poorly expressed, whereas neurons showed a very high sensitivity to the toxic effect of glutamate. Measurements of the concentration of intracellular free calcium ions ([Ca2+]i) showed that the STZ-induced [Ca2+]i increase in young neurons was six times higher than that in mature neurons. Our results show that STZ is very toxic for immature neurons and probably it can significantly impair neurogenesis. |
| Databáze: | OpenAIRE |
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