Co-activation of synovial fibroblasts by laminin-111 and transforming growth factor-β induces expression of matrix metalloproteinases 3 and 10 independently of nuclear factor-κB
| Autor: | K, Warstat, T, Pap, G, Klein, S, Gay, W K, Aicher |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Immunology
Matrix metalloproteinase Matrix (biology) General Biochemistry Genetics and Molecular Biology Laminin 111 Arthritis Rheumatoid Matrix Metalloproteinase 10 Rheumatology Transforming Growth Factor beta Laminin Osteoarthritis medicine Humans Immunology and Allergy RNA Messenger Phosphorylation Fibroblast Cells Cultured Early Growth Response Protein 1 biology Reverse Transcriptase Polymerase Chain Reaction Synovial Membrane NF-kappa B Tissue Inhibitor of Metalloproteinases Fibroblasts Molecular biology medicine.anatomical_structure Gene Expression Regulation biology.protein Electrophoresis Polyacrylamide Gel Matrix Metalloproteinase 3 Synovial membrane Signal transduction Proto-Oncogene Proteins c-fos Transforming growth factor |
| Zdroj: | Annals of the Rheumatic Diseases. 67:559-562 |
| ISSN: | 1468-2060 0003-4967 |
| DOI: | 10.1136/ard.2007.073809 |
| Popis: | We showed previously that the attachment of synovial fibroblasts to laminin (LM)-111 in the presence of transforming growth factor-β induces significant expression of the matrix metalloproteinase (MMP)-3. Here we go on to investigate the regulation of additional MMPs and their specific tissue inhibitors of matrix proteases (TIMPs). Changes in steady-state mRNA levels encoding TIMPs and MMPs were investigated by quantitative reverse transcription–polymerase chain reaction. Production of MMPs was monitored by a multiplexed immunoarray. Signal transduction pathways were studied by immunoblotting. Attachment of synovial fibroblasts to LM-111 in the presence of transforming growth factor-β induced significant increases in MMP-3 mRNA (12.35-fold, p |
| Databáze: | OpenAIRE |
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