Selenotranscriptomic Analyses Identify Signature Selenoproteins in Brain Regions in a Mouse Model of Parkinson's Disease

Autor: Jing Zheng, Wen-Hsing Cheng, Song-Fang Chen, Hui-Hui Fan, Sheng-Nan Sun, Hui Zhu, Yang-Lie Ye, Jian-Hong Zhu, Xiong Zhang, Hong-Mei Wu
Rok vydání: 2016
Předmět:
0301 basic medicine
Pathology
Cerebellum
Parkinson's disease
SEP15
lcsh:Medicine
Gene Expression
GPX4
Hippocampus
Biochemistry
0302 clinical medicine
Animal Cells
Medicine and Health Sciences
lcsh:Science
chemistry.chemical_classification
Cerebral Cortex
Neurons
Multidisciplinary
Movement Disorders
integumentary system
Brain
Neurodegenerative Diseases
Parkinson Disease
Neurochemistry
Animal Models
Substantia Nigra
medicine.anatomical_structure
Neurology
Anatomy
Neurochemicals
Cellular Types
Research Article
medicine.medical_specialty
SEPP1
Substantia nigra
Mouse Models
Biology
Research and Analysis Methods
03 medical and health sciences
Model Organisms
Internal medicine
medicine
Genetics
lcsh:R
Biology and Life Sciences
Cell Biology
medicine.disease
Pons
030104 developmental biology
Endocrinology
chemistry
Cellular Neuroscience
lcsh:Q
Selenoprotein
Dopaminergics
030217 neurology & neurosurgery
Neuroscience
Zdroj: PLoS ONE
PLoS ONE, Vol 11, Iss 9, p e0163372 (2016)
ISSN: 1932-6203
Popis: Genes of selenoproteome have been increasingly implicated in various aspects of neurobiology and neurological disorders, but remain largely elusive in Parkinson's disease (PD). In this study, we investigated the selenotranscriptome (24 selenoproteins in total) in five brain regions (cerebellum, substantia nigra, cortex, pons and hippocampus) by real time qPCR in a two-phase manner using a mouse model of chronic PD. A wide range of changes in selenotranscriptome was observed in a manner depending on selenoproteins and brain regions. While Selv mRNA was not detectable and Dio1& 3 mRNA levels were not affected, 1, 11 and 9 selenoproteins displayed patterns of increase only, decrease only, and mixed response, respectively, in these brain regions of PD mice. In particular, the mRNA expression of Gpx1-4 showed only a decreased trend in the PD mouse brains. In substantia nigra, levels of 17 selenoprotein mRNAs were significantly decreased whereas no selenoprotein was up-regulated in the PD mice. In contrast, the majority of selenotranscriptome did not change and a few selenoprotein mRNAs that respond displayed a mixed pattern of up- and down-regulation in cerebellum, cortex, hippocampus, and/or pons of the PD mice. Gpx4, Sep15, Selm, Sepw1, and Sepp1 mRNAs were most abundant across all these five brain regions. Our results showed differential responses of selenoproteins in various brain regions of the PD mouse model, providing critical selenotranscriptomic profiling for future functional investigation of individual selenoprotein in PD etiology.
Databáze: OpenAIRE
Externí odkaz: