Myo-inositol oxygenase from oat seedlings
| Autor: | O. Hoffmann-Ostenhof, Elisabeth Koller, Franz Koller |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
chemistry.chemical_classification
Oxygenase Clinical chemistry Clinical Biochemistry Rat kidney Ether Cell Biology General Medicine Hydrogen-Ion Concentration Plants Biology Yeast Inositol oxygenase Molecular Weight Kinetics Structure-Activity Relationship chemistry.chemical_compound Enzyme chemistry Biochemistry Oxygenases Inositol Edible Grain Molecular Biology |
| Zdroj: | Molecular and Cellular Biochemistry. 10:33-39 |
| ISSN: | 1573-4919 0300-8177 |
| DOI: | 10.1007/bf01731679 |
| Popis: | Enzyme preparations from oat seedlings showing the activity of myo-inositol oxygenase (E.C.1.13.99.1) have been described previously. In contrast to myo-inositol oxygenase preparations from other sources, e.g. rat kidney or yeast, the oat enzyme seemed to exhibit a somewhat less stringent activity, acting on other inositols and inositol methyl ethers as well as on myo-inositol. By purification of the enzyme present in the extract from oat seedlings with the help of an affinity gel specific for enzymes acting on myo-inositol a homogeneous enzyme preparation was obtained, which shows the same strict specificity as the myo-inositol oxygenase from other sources. It has a molecular weight of 62,000 and tends to aggregate to oligomers (up to tetramers) under physiological pH-values; in more alkaline media dissociation to monomers is observed. The action on the other inositols and inositol methyl ethers is apparently due to one or more other enzymes, which are also adsorbed on the affinity gel, but can be separated from the myo-inositol oxygenase by elution with increasing concentrations of myo-inositol. |
| Databáze: | OpenAIRE |
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