Effect of superposed electromagnetic noise on DNA damage of lens epithelial cells induced by microwave radiation
| Autor: | Jiliang He, Yibo Yu, Kai-jun Wang, Ke Yao, Qunli Zeng, Wei Wu, Deqiang Lu |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Cell cycle checkpoint
Electromagnetics DNA damage Apoptosis Biology Flow cytometry Cell Line chemistry.chemical_compound Electromagnetic Fields Lens Crystalline medicine Humans Fluorescent Antibody Technique Indirect Microwaves Genetics medicine.diagnostic_test Cell Cycle Epithelial Cells DNA Cell cycle Flow Cytometry Histone chemistry Microscopy Fluorescence Biophysics biology.protein Comet Assay Reactive Oxygen Species DNA Damage |
| Zdroj: | Investigative ophthalmologyvisual science. 49(5) |
| ISSN: | 0146-0404 |
| Popis: | To investigate the influence of the 1.8-GHz radiofrequency fields (RFs) of the Global System for Mobile Communications on DNA damage, intracellular reactive oxygen species (ROS) formation, cell cycle, and apoptosis in human lens epithelial cells (hLECs) and whether the effects induced by RF could be blocked by superposing of electromagnetic noise.After 24-hour intermittent exposure at the specific absorption rate of 1 W/kg, 2 W/kg, 3 W/kg, and 4 W/kg, the DNA damage of hLECs was examined by alkaline comet assay and immunofluorescence microscope detection of the phosphorylated form of histone variant H2AX (gammaH2AX) foci, respectively. ROS production was quantified by the fluorescent probe 2',7'-dichlorodihydrofluorescein diacetate (DCFH-DA). Cell cycle and cell apoptosis were determined by flow cytometry.DNA damage examined by alkaline comet assay was significantly increased after 3 W/kg and 4 W/kg radiation (P0.05), whereas the double-strand breaks (DSBs) evaluated by gammaH2AX foci were significantly increased only after 4 W/kg radiation (P0.05). Significantly elevated intracellular ROS levels were also detected in the 3-W/kg and 4-W/kg groups (P0.05). After exposure to 4 W/kg for 24 hours, hLECs exhibited significant G(0)/G(1) arrest (P0.05). There was no detectable difference in cell apoptosis between the microwave radiation and sham exposure groups (P0.05). All the effects mentioned were blocked when the RF was superposed with 2 muT electromagnetic noise.Microwave radiation induced hLEC DNA damage after G(0)/G(1) arrest does not lead to cell apoptosis. The increased ROS observed may be associated with DNA damage. Superposed electromagnetic noise blocks microwave radiation-induced DNA damage, ROS formation, and cell cycle arrest. |
| Databáze: | OpenAIRE |
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