Pomegranate peel induced biogenic synthesis of silver nanoparticles and their multifaceted potential against intracellular pathogen and cancer
Autor: | Tanveer A. Wani, Amer M. Alanazi, Nawaf A. Alsaif, Mashooq A. Bhat, Azmat Ali Khan |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
chemistry.chemical_classification
Reactive oxygen species Chemistry QH301-705.5 Biofilm Apoptosis medicine.disease_cause Listeria monocytogenes Silver nanoparticle Green synthesis chemistry.chemical_compound Biochemistry medicine DNA fragmentation Original Article Viability assay Crystal violet Biology (General) Silver nanoparticles General Agricultural and Biological Sciences Cancer |
Zdroj: | Saudi Journal of Biological Sciences Saudi Journal of Biological Sciences, Vol 28, Iss 8, Pp 4191-4200 (2021) |
ISSN: | 2213-7106 1319-562X |
Popis: | In the field of nano-biotechnology, silver nanoparticles (AgNPs) share a status of high repute owing to their remarkable medicinal values. Biological synthesis of environment-friendly AgNPs using plant extracts has emerged as the beneficial alternative approach to chemical synthesis. In the current study, we have synthesized biogenic silver nanoparticles (PG-AgNPs) using the peel extract of Punica granatum as a reducing and stabilizing agent. The as-synthesized PG-AgNPs were characterized and evaluated for their antibacterial and anticancer potential. UV-Visible spectroscopy, transmission electron microscopy (TEM) and dynamic light scattering (DLS) confirmed the formation of biogenic PG-AgNPs. The antibacterial potential was assessed against the biofilm of Listeria monocytogenes. The PG-AgNPs were efficacious against sessile bacteria and their biofilm as well. The as-synthesized nanoparticles at sub-MIC values showed dose-dependent inhibition of biofilm formation. Corroborating results were observed under crystal violet assay, Congo red staining, Confocal microscopy and SEM analysis. The anticancer ability of the nanoparticles was evaluated against MDA-MB-231 metastatic breast cancer cells. As evident from the MTT results, PG-AgNPs significantly reduced the cell viability in a dose-dependent manner. Exposure of MDA-MB-231 cells led to the accumulation of reactive oxygen species (ROS). Morphological changes and DNA fragmentation showed the strong positive effect of PG-AgNPs on the induction of apoptosis. Collectively, the as-synthesized PG-AgNPs evolved with synergistically emerged attributes that were effective against L. monocytogenes and also inhibited its biofilm formation; moreover, the system displayed lower cytotoxic manifestation towards mammalian cells. In addition, the PG-AgNPs embodies intriguing anticancer potential against metastatic breast cancer cells. |
Databáze: | OpenAIRE |
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