Replication of equine herpesvirus type 1 in freshly isolated equine peripheral blood mononuclear cells and changes in susceptibility following mitogen stimulation
| Autor: | Wim Buddaert, Karen van der Meulen, Hans Nauwynck, Maurice Pensaert |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
education.field_of_study
Population Biology Lymphocyte Activation Virus Replication Microbiology Virology Peripheral blood mononuclear cell Monocytes In vitro Virus Antigen Viral replication Leukocytes Mononuclear Extracellular Animals Horses Lymphocytes Mitogens education Antigens Viral Pan-T antigens Cells Cultured Herpesvirus 1 Equid |
| Zdroj: | Microbiology. 81:21-25 |
| ISSN: | 1465-2080 1350-0872 |
| DOI: | 10.1099/0022-1317-81-1-21 |
| Popis: | In the present study, the outcome of an inoculation of equine peripheral blood mononuclear cells (PBMC) with equine herpesvirus type 1 (EHV-1) was studiedin vitro. Cytoplasmic and plasma membrane expression of viral antigens, intra- and extracellular virus titres, and plaque formation in co-culture were determined. EHV-1 replicated in monocytes, although in a highly restricted way. Viral antigens were found at maximum levels (8·7% of the monocytes) at 12 h post-infection. The infection was productive in 0·16% of the monocytes. The virus yield was 100·7TCID50per productive cell. In a population of resting lymphocytes, 0·9% of cells were infected and less than 0·05% produced infectious virus. After prestimulation with different mitogens, the number of infected lymphocytes increased four to twelve times. The susceptible lymphocytes were T-lymphocytes. In mitogen-stimulated lymphocytes, clear expression of viral antigens was found on the plasma membrane. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|