IR-MALDI ion mobility spectrometry
| Autor: | José Villatoro, Martin Zühlke, Hans-Gerd Löhmannsröben, Toralf Beitz, Daniel Riebe, Jens Riedel |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Ions
Delayed extraction Resolution (mass spectrometry) Infrared Rays Ion-mobility spectrometry Chemistry Lasers 010401 analytical chemistry Analytical chemistry Equipment Design 010402 general chemistry Mass spectrometry 01 natural sciences Biochemistry Ion source 0104 chemical sciences Analytical Chemistry Ion Ion-mobility spectrometry–mass spectrometry Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Ion Mobility Spectrometry Institut für Chemie Time-of-flight mass spectrometry |
| Popis: | The novel combination of infrared matrix-assisted laser dispersion and ionization (IR-MALDI) with ion mobility (IM) spectrometry makes it possible to investigate biomolecules in their natural environment, liquid water. As an alternative to an ESI source, the IR-MALDI source was implemented in an in-house-developed ion mobility (IM) spectrometer. The release of ions directly from an aqueous solution is based on a phase explosion, induced by the absorption of an IR laser pulse (lambda = 2.94 mu m, 6 ns pulse width), which disperses the liquid as nano- and micro-droplets. The prerequisites for the application of IR-MALDI-IM spectrometry as an analytical method are narrow analyte ion signal peaks for a high spectrometer resolution. This can only be achieved by improving the desolvation of ions. One way to full desolvation is to give the cluster ions sufficient time to desolvate. Two methods for achieving this are studied: the implementation of an additional drift tube, as in ESI-IM-spectrometry, and the delayed extraction of the ions. As a result of this optimization procedure, limits of detection between 5 nM and 2.5 mu M as well as linear dynamic ranges of 2-3 orders of magnitude were obtained for a number of substances. The ability of this method to analyze simple mixtures is illustrated by the separation of two different surfactant mixtures. |
| Databáze: | OpenAIRE |
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