Oxidative stress and neurodegeneration in penile ischaemia
| Autor: | Mike B. Siroky, Subbarao V. Yalla, Kazem M. Azadzoi, Tomasz Golabek, Ziv Radisavljevic |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Male
Pathology medicine.medical_specialty Endothelium Urology Ischemia Blood Pressure Erectile tissue medicine.disease_cause Impotence Vasculogenic Superoxide dismutase Random Allocation chemistry.chemical_compound medicine Animals biology business.industry Nitrotyrosine Neurodegeneration medicine.disease Immunohistochemistry Oxidative Stress medicine.anatomical_structure Erectile dysfunction chemistry Nerve Degeneration biology.protein Rabbits business Biomarkers Blood Flow Velocity Oxidative stress Penis |
| Zdroj: | BJU International. 105:404-410 |
| ISSN: | 1464-410X 1464-4096 |
| DOI: | 10.1111/j.1464-410x.2009.08717.x |
| Popis: | OBJECTIVE To seek markers of oxidative stress and examine neural structural integrity in chronic penile ischaemia using a rabbit model of arteriogenic erectile dysfunction (ED), as the role of ischaemia in penile neuropathy and the oxidative mechanism of neurodegeneration in ED remains unknown. MATERIALS AND METHODS A rabbit model of atherosclerosis-induced ED was developed by partial balloon de-endothelialization of the iliac arteries. After 10 weeks, intracavernosal blood flow and erectile function in the arteriogenic ED group were compared with age-matched controls. Erectile tissues were processed for analysis of oxidative stress markers and nerve fibre density using enzyme immunoassay and immunohistochemical staining, respectively. Oxidative stress-sensitive genes were determined with quantitative real-time polymerase chain reaction. Tissue ultrastructure was examined by transmission electron microscopy. RESULTS Significant erectile tissue ischaemia, erectile dysfunction, increased levels of oxidative products, and marked nitrotyrosine immunoreactivity was evident in the ED group. Oxidative stress-sensitive genes encoding hypoxia inducible factor-1α (HIF-1α), superoxide dismutase (SOD), aldose reductase (AR) and nerve growth factor (NGF) were up-regulated in the ischaemic erectile tissue. These changes were associated with collapsed axonal and Schwann cell profiles, neurodegeneration, mitochondrial structural damage, increased caveolae, loss of endothelium, and sporadic vacuolization. CONCLUSIONS Neuropathy appears to follow the vascular insult in arteriogenic ED. Neural injury in penile ischaemia involves a neurovascular phenomenon mediated by oxidative free radicals. Mitochondrial structural damage and increased HIF-1α gene expression may be early signals of oxidative stress and neurodegeneration in ED. Up-regulation of SOD, AR and NGF may be a coordinated defensive reaction to oxidative radicals that seems to fail to prevent neural injury in the ischaemic penis. Our study introduces the concept of oxidative neurodegeneration in the pathophysiology of arteriogenic ED. Therapeutic strategies to protect penile nerves from free radical incursion may enhance the efficacy of surgical and pharmacological interventions in arteriogenic ED. |
| Databáze: | OpenAIRE |
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