Mutual chemical effect of autograft and octacalcium phosphate implantation on enhancing intramembranous bone regeneration
| Autor: | Osamu Suzuki, Hisashi Ozaki, Kaori Tsuchiya, Yukari Shiwaku, Susumu Sakai, Ryo Hamai |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
30 Bio-inspired and biomedical materials
autograft octacalcium phosphate mechanical mixing dissolution Calvaria 02 engineering and technology 010402 general chemistry 01 natural sciences chemistry.chemical_compound bone regeneration medicine General Materials Science mineralization Bone regeneration Octacalcium phosphate Materials of engineering and construction. Mechanics of materials Regeneration (biology) Mineralization (soil science) 021001 nanoscience & nanotechnology Autologous bone Focus on Trends in Biomaterials in Japan 0104 chemical sciences medicine.anatomical_structure chemistry Intramembranous ossification Biophysics TA401-492 intramembranous bone 0210 nano-technology 107 Glass and ceramic materials complex TP248.13-248.65 Research Article autologous bone Biotechnology |
| Zdroj: | Science and Technology of Advanced Materials, Vol 22, Iss 1, Pp 345-362 (2021) Science and Technology of Advanced Materials article-version (VoR) Version of Record |
| ISSN: | 1878-5514 1468-6996 |
| Popis: | This study examined the effect of a mixture of octacalcium phosphate (OCP) and autologous bone on bone regeneration in rat calvaria critical-sized defect (CSD). Mechanically mixed OCP and autologous bone granules (OCP+Auto), approximately 500 to 1000 μm in diameter, and each individual material were implanted in rat CSD for 8 weeks, and subjected to X-ray micro-computed tomography (micro-CT), histology, tartrate-resistant acid phosphatase (TRAP) staining, and histomorphometry for bone regeneration. Osteoblastic differentiation from mesenchymal stem cells (D1 cells) was examined in the presence of non-contacting materials by alkaline phosphatase (ALP) activity for 21 days. The material properties and medium composition before and after the incubation were determined by selected area electron diffraction (SAED) under transmission electron microscopy (TEM), Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM), and chemical analysis. The results showed that while bone formation coupled with TRAP-positive osteoclastic resorption and cellular ALP activity were the highest in the Auto group, a positive effect per OCP weight or per autologous bone weight on ALP activity was found. Although the OCP structure was maintained even after the incubation (SAED), micro-deposits were grown on OCP surfaces (TEM). Fibrous tissue was also exposed on the autologous bone surfaces (SEM). Through FT-IR absorption, it was determined that bone mineral-like characteristics of the phosphate group increased in the OCP + Auto group. These findings were interpreted as a structural change from OCP to the apatitic phase, a conclusion supported by the medium degree of saturation changes. The results demonstrate the mutual chemical effect of mixing OCP with autologous bone as an active bone substitute material. Graphical abstract |
| Databáze: | OpenAIRE |
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