Binding of Bisphenol-F, a bisphenol analogue, to calf thymus DNA by multi-spectroscopic and molecular docking studies
| Autor: | Afia Usman, Masood Ahmad |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
030103 biophysics Circular dichroism Environmental Engineering Bisphenol Stereochemistry Health Toxicology and Mutagenesis Endocrine Disruptors 010402 general chemistry 01 natural sciences 03 medical and health sciences chemistry.chemical_compound Plasmid Phenols Ethidium Environmental Chemistry Animals Benzhydryl Compounds DNA Cleavage Quenching (fluorescence) Binding Sites Hydrogen bond Spectrum Analysis Public Health Environmental and Occupational Health Hydrogen Bonding General Medicine General Chemistry DNA Pollution 0104 chemical sciences Molecular Docking Simulation chemistry Bisbenzimidazole Thermodynamics Cattle Cyclic voltammetry Ethidium bromide Mutagens |
| Zdroj: | Chemosphere. 181 |
| ISSN: | 1879-1298 |
| Popis: | BPF (Bisphenol-F), a member of the bisphenol family, having a wide range of industrial applications is gradually replacing Bisphenol-A. It is a recognized endocrine disrupting chemical (EDC). EDCs have been implicated in increased incidences of breast, prostate and testis cancers besides diabetes, obesity and decreased fertility. Due to the adverse effects of EDCs on human health, attempts have been directed towards their mechanism of toxicity especially at the molecular level. Hence, to understand the mechanism at the DNA level, interaction of BPF with calf thymus DNA was studied employing multi-spectroscopic, voltammetric and molecular docking techniques. Fluorescence spectra, cyclic voltammetry (CV), circular dichroism (CD) and molecular docking studies of BPF with DNA were suggestive of minor groove binding of BPF. UV–visible absorption and fluorescence spectra suggested static quenching due to complex formation between BPF and ctDNA. Hoechst 33258 (HO) and ethidium bromide (EB) displacement studies further confirmed such mode of BPF interaction. Thermodynamic and molecular docking parameters revealed the mechanism of binding of BPF with ctDNA to be favorable and spontaneous due to negative ΔG and occurring through hydrogen bonds and van der waals interactions. BPF induced DNA cleavage under in vitro conditions by plasmid nicking assay suggested it to be genotoxic. |
| Databáze: | OpenAIRE |
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