Effects of disease activity on anti-Saccharomyces cerevisiae antibodies - Implications for diagnosis and follow-up of children with Crohn's disease
| Autor: | M. T. Romano, G. Polito, Massimo Fontana, Luigi Greco, Gianluigi De Angelis, Roberto Berni Canani, Gianluca Terrin, Simona Fecarotta, Concetta Sferlazzas, Graziella Guariso, Salvatore Cucchiara, Paola Roggero, Arrigo Barabino |
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| Přispěvatelé: | BERNI CANANI, Roberto, Romano, Mt, Greco, Luigi, Terrin, G, Sferlazzas, C, Barabino, A, Fontana, M, Roggero, P, Guariso, G, DE ANGELIS, G, Fecarotta, S, Polito, G, Cucchiara, S. |
| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
Immunoglobulin A
Adult Male Adolescent crohn's disease Disease Saccharomyces cerevisiae Inflammatory bowel disease Sensitivity and Specificity Disease activity Diagnosis Differential Crohn Disease children inflammatory bowel disease Immunology and Allergy Medicine Humans anti-saccharomyces cerevisiae antibody Child Antibodies Fungal Crohn's disease biology business.industry Gastroenterology Anti–Saccharomyces cerevisiae antibody Infant disease activity medicine.disease Inflammatory Bowel Diseases Titer Case-Control Studies Child Preschool Immunology biology.protein Female Antibody business |
| Popis: | Background: To determine diagnostic accuracy of anti–Saccharomyces cerevisiae antibodies (ASCA) in identifying children with inflammatory bowel disease (IBD) and to differentiate Crohn's disease (CD) from other IBD forms; and to determine the effect of medical or surgical treatment and of disease location and activity on ASCA titers. Methods: Serum samples were obtained from 196 IBD children and 142 controls. ASCA IgA and IgG titers were measured by ELISA. Measurements were repeated during the follow up of CD children. Results: ASCA titers were significantly higher in CD than in other IBD and in control patients. Combination of IgA and IgG ASCA positivity was highly specific for CD. Medical treatment and disease location did not influence assay results. Significantly lower ASCA titers were obtained in CD children with intestinal resection compared to CD-affected children who did not undergo surgical resection. ASCA titers correlated significantly with disease activity, and children with severe active disease showed higher ASCA values compared to those in remission. A signficant reduction of ASCA was observed during the follow-up of CD children when clinical remission was achieved. Conclusions: The diagnostic accuracy of ASCA is influenced by disease activity and this suggests an additional use for the follow-up of CD children of this assay. |
| Databáze: | OpenAIRE |
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