Lymphatic invasion using D2-40 monoclonal antibody and its relationship to lymph node micrometastasis in pN0 gastric cancer
| Autor: | Hokita S, Sumiya Ishigami, Shoji Natsugoe, Shigehiro Yanagida, Hideo Arima, Yukou Mataki, Takaaki Arigami, Takashi Aikou, Katsuhiko Ehi, Yoshikazu Uenosono |
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| Rok vydání: | 2005 |
| Předmět: |
Male
Cancer Research Pathology medicine.medical_specialty reverse transcription–polymerase chain reaction Lymphovascular invasion Biology Immunoenzyme Techniques lymphatic invasion Stomach Neoplasms medicine Humans Neoplasm Invasiveness Stomach cancer Molecular Diagnostics Lymph node Aged Neoplasm Staging Aged 80 and over Reverse Transcriptase Polymerase Chain Reaction gastric cancer Micrometastasis Antibodies Monoclonal Cancer Middle Aged medicine.disease lymph node micrometastasis medicine.anatomical_structure Lymphatic system Oncology Lymphatic Metastasis immunohistochemistry Immunohistochemistry Female Lymph Nodes Lymph Endothelium Lymphatic |
| Zdroj: | British Journal of Cancer |
| ISSN: | 1532-1827 0007-0920 |
| DOI: | 10.1038/sj.bjc.6602739 |
| Popis: | The monoclonal antibody D2-40 is a specific lymphatic endothelial markers and D2-40 staining have been applicable to evaluate lymphatic invasion in various malignant neoplasms. In the present study, we investigated lymph node micrometastasis determined by immunohistochemistry (IHC) and reverse transcription–polymerase chain reaction (RT–PCR) in all dissected lymph nodes obtained from 80 patients with node-negative gastric cancer, and analysed the relationship between micrometastasis and clinicopathological findings including lymphatic invasion of the resected primary tumour using D2-40 immunohistochemical staining. The incidence of micrometastasis determined by IHC and RT–PCR was 11.3% (nine out of 80) and 31.3% (25 out of 80), respectively. Although haematoxylin–eosin (HE) staining revealed lymphatic invasion in 11.3% (nine out of 80) of patients, D2-40 staining uncovered new invasion in 23.8% (19 out of 80) of patients. In the diagnosis of HE and D2-40 staining, the incidence of micrometastasis was significantly higher in patients with lymphatic invasion than in those without lymphatic invasion (P=0.0150 and P |
| Databáze: | OpenAIRE |
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