Effect of HSP65 on the Expression of Adhesion Molecules in Mice Heart Endothelial Cells
| Autor: | Changjiang Sun, Juan Yu, Jianxin Wang, Huoyan Ji |
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| Rok vydání: | 2011 |
| Předmět: |
Lipopolysaccharides
Immunology Mice Animals Immunology and Allergy Inducer RNA Messenger Cells Cultured Heat-Shock Proteins Inflammation Messenger RNA Cell adhesion molecule Chemistry Myocardium Soluble cell adhesion molecules Endothelial Cells Adhesion Intercellular Adhesion Molecule-1 Intercellular adhesion molecule In vitro Cell biology P-Selectin Animals Newborn Neural cell adhesion molecule Amine Oxidase (Copper-Containing) E-Selectin Cell Adhesion Molecules |
| Zdroj: | Inflammation. 35:1049-1057 |
| ISSN: | 1573-2576 0360-3997 |
| Popis: | This study aims to research the effect of HSP65 on the expression of adhesion molecules in activated mice heart endothelial cells (MHECs), which were from myocardial tissue of newborn animals. We used different concentrations of LPS as potent inducers to stimulate MHECs, adhesion molecule expression in vitro, including intercellular adhesion molecule-1 (ICAM-1), vascular adhesion molecule-1 (VCAM-1), E-, and P-selectins, then compared the mRNA and protein levels of adhesion molecules expression with or without HSP65 treatment at different levels. The optimal concentration of LPS to induce MHECs adhesion molecule expression is 100 ng/ml; HSP65 treatment significantly reduced the mRNA and protein levels of MHECs’ ICAM-1, VCAM-1, E-, and P-selectins expression (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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