Betel Nut Arecoline Induces Different Phases of Growth Arrest between Normal and Cancerous Prostate Cells through the Reactive Oxygen Species Pathway

Autor: Yen Yue Lin, Chih Pin Chuu, Jing Yao Jheng, Jia Yu Wang, Yi Wei Tsuei, Yung Hsi Kao, Yow Chii Kuo, An Ci Siao, Li Jane Shih
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Cyclin-Dependent Kinase Inhibitor p21
Male
Cell Survival
Arecoline
Cell Cycle Proteins
urologic and male genital diseases
Article
Catalysis
Inorganic Chemistry
lcsh:Chemistry
Cyclin D1
cyclin
Cyclin-dependent kinase
LNCaP
medicine
Humans
Physical and Theoretical Chemistry
Cyclin D3
Cyclin B1
Molecular Biology
lcsh:QH301-705.5
Areca
Spectroscopy
reactive oxygen species
Cyclin-dependent kinase 1
Dose-Response Relationship
Drug
biology
p21
Chemistry
areca nut
Organic Chemistry
Prostatic Neoplasms
Cell Cycle Checkpoints
General Medicine
Cell cycle
prostate cancer
Computer Science Applications
Gene Expression Regulation
Neoplastic
cyclin-dependent kinase
lcsh:Biology (General)
lcsh:QD1-999
Cancer research
biology.protein
cell cycle
biological phenomena
cell phenomena
and immunity
Signal Transduction
medicine.drug
Zdroj: International Journal of Molecular Sciences, Vol 21, Iss 9219, p 9219 (2020)
International Journal of Molecular Sciences
Volume 21
Issue 23
ISSN: 1661-6596
1422-0067
Popis: Prostate cancer (PCa) is a reproductive system cancer in elderly men. We investigated the effects of betel nut arecoline on the growth of normal and cancerous prostate cells. Normal RWPE-1 prostate epithelial cells, androgen-independent PC-3 PCa cells, and androgen-dependent LNCaP PCa cells were used. Arecoline inhibited their growth in dose- and time-dependent manners. Arecoline caused RWPE-1 and PC-3 cell cycle arrest in the G2/M phase and LNCaP cell arrest in the G0/G1 phase. In RWPE-1 cells, arecoline increased the expression of cyclin-dependent kinase (CDK)-1, p21, and cyclins B1 and D3, decreased the expression of CDK2, and had no effects on CDK4 and cyclin D1 expression. In PC-3 cells, arecoline decreased CDK1, CDK2, CDK4, p21, p27, and cyclin D1 and D3 protein expression and increased cyclin B1 protein expression. In LNCaP cells, arecoline decreased CDK2, CDK4, and cyclin D1 expression
increased p21, p27, and cyclin D3 expression
had no effects on CDK1 and cyclin B1 expression. The antioxidant N-acetylcysteine blocked the arecoline-induced increase in reactive oxygen species production, decreased cell viability, altered the cell cycle, and changed the cell cycle regulatory protein levels. Thus, arecoline oxidant exerts differential effects on the cell cycle through modulations of regulatory proteins.
Databáze: OpenAIRE
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