Production of alpha-amylase and glucoamylase by a new isolate of Trichoderma sp. using sorghum starch as a carbon source
| Autor: | Laís Calissi Brisolla Tavares, T. C. Vessoni Penna, Sunao Sato, Attilio Converti, R. A. Pacheco-Chavez, João Carlos Monteiro de Carvalho |
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| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
Trichoderma sp
Environmental Engineering biology Starch Microorganism food and beverages Bioengineering Fungi imperfecti Sorghum starch Sorghum biology.organism_classification Hydrolysis chemistry.chemical_compound alpha-Amylase chemistry Biochemistry Glucoamylase Trichoderma biology.protein Amylase Food science Incubation Biotechnology |
| Popis: | The aim of this work is to evaluate the performance of a new Trichoderma sp. isolate to produce extracellular α-amylase and glucoamylase from raw sorghum starch. To reduce the costs of starch saccharification and the consumption of amylolytic enzymes, this microorganism has been used for the first time in cultivations using such a carbon source without any prior gelatinization. Incubation of this microorganism at 28 °C, 180 rpm and starting pH 5.3 ensured α-amylase and glucoamylase activities of 258±2.8 and 83±1.2 U/L after 24 and 120 h, respectively, and maximum productivities of 11±0.9 and 2.3±0.2 U/Lh, after 24 h. In general, α-amylase was produced 4-5 times more quickly than glucoamylase and no less than 78 % of starting sorghum starch was hydrolyzed, releasing 49±7 mg/L total reducing sugars and 28±3 mg/L glucose. It is the first time that an isolate of the Trichoderma genus was found to express such amylolytic activities using raw sorghum starch. The ability of this microorganism to overproduce amylases could be usefully exploited for direct saccharification of other raw starches using different nitrogen sources. |
| Databáze: | OpenAIRE |
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