Sensitivity and specificity of the AdenoPlus test for diagnosing adenoviral conjunctivitis
| Autor: | Christopher E. Starr, Thomas Boland, Michael DellaVecchia, Marguerite B. McDonald, William Trattler, Robert Sambursky, Shachar Tauber, Jodi Luchs, Murray Friedberg |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Adult
Male medicine.medical_specialty Virus Cultivation Adolescent medicine.drug_class Antibiotics Viral Conjunctivitis Fluorescent Antibody Technique Diagnostic Techniques Ophthalmological Immunofluorescence Gastroenterology Polymerase Chain Reaction Sensitivity and Specificity law.invention Adenovirus Infections Human Conjunctivitis Viral Young Adult Randomized controlled trial Double-Blind Method law Predictive Value of Tests Internal medicine medicine Humans False Positive Reactions Prospective Studies Prospective cohort study Child Antigens Viral Polymerase chain reaction Aged Aged 80 and over medicine.diagnostic_test business.industry Adenoviruses Human Reproducibility of Results Middle Aged Clinical trial Ophthalmology Predictive value of tests Child Preschool Tears Immunology Acute Disease DNA Viral Female business |
| Zdroj: | JAMA ophthalmology. 131(1) |
| ISSN: | 2168-6173 |
| Popis: | Objective To compare the clinical sensitivity and specificity of the AdenoPlus test with those of both viral cell culture (CC) with confirmatory immunofluorescence assay (IFA) and polymerase chain reaction (PCR) at detecting the presence of adenovirus in tear fluid. Methods A prospective, sequential, masked, multicenter clinical trial enrolled 128 patients presenting with a clinical diagnosis of acute viral conjunctivitis from a combination of 8 private ophthalmology practices and academic centers. Patients were tested with AdenoPlus, CC-IFA, and PCR to detect the presence of adenovirus. Main Outcome Measures The sensitivity and specificity of AdenoPlus were assessed for identifying cases of adenoviral conjunctivitis. Results Of the 128 patients enrolled, 36 patients' results were found to be positive by either CC-IFA or PCR and 29 patients' results were found to be positive by both CC-IFA and PCR. When compared only with CC-IFA, AdenoPlus showed a sensitivity of 90% (28/31) and specificity of 96% (93/97). When compared only with PCR, AdenoPlus showed a sensitivity of 85% (29/34) and specificity of 98% (89/91). When compared with both CC-IFA and PCR, AdenoPlus showed a sensitivity of 93% (27/29) and specificity of 98% (88/90). When compared with PCR, CC-IFA showed a sensitivity of 85% (29/34) and specificity of 99% (90/91). Conclusion AdenoPlus is sensitive and specific at detecting adenoviral conjunctivitis. Application to Clinical Practice An accurate and rapid in-office test can prevent the misdiagnosis of adenoviral conjunctivitis that leads to the spread of disease, unnecessary antibiotic use, and increased health care costs. Additionally, AdenoPlus may help a clinician make a more informed treatment decision regarding the use of novel therapeutics. Trial Registration clinicaltrials.gov Identifier: NCT00921895 |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|